4.3 Article

The complete chloroplast genome of Cycas bifida, an extremely small population protected species

Journal

MITOCHONDRIAL DNA PART B-RESOURCES
Volume 6, Issue 10, Pages 2960-2961

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/23802359.2021.1973919

Keywords

Cycas bifida; chloroplast genome Illumina sequencing endangered species

Funding

  1. Fundamental Research Fund of Guangxi Institute of Botany [GZY 21007]
  2. Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain [20-065-7]

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This study reported the first chloroplast genome sequence of the rare plant Cycas bifida in China, revealing that it contains 131 genes and is closely related to Cycas szechuanensis.
Cycas bifida (Dyer) K.D.Hill (2004) is an extremely small population-protected species of China. In this study, we reported the first chloroplast genome sequence of C. bifida. The chloroplast genome of C. bifida included two single-copy regions (large single-copy (LSC) and small single-copy (SSC)) and a pair of inverted repeats (IRs) regions comprising 88,946 bp, 23,107 bp, and 25,053 bp, respectively. The complete chloroplast genome of C. bifida contains 131 genes, including 86 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. The overall GC content of the C. bifida chloroplast genome is 39.41%, and the LSC, SSC, and IR regions occupy 38.70%, 36.52%, and 42.02%, respectively. A phylogenetic analysis was performed based on complete chloroplast genomes from 15 species and found that C. bifida was closely related to Cycas szechuanensis W.C.Cheng & L.K.Fu.

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