Journal
AGRONOMY-BASEL
Volume 11, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/agronomy11051009
Keywords
Bradyrhizobium japonicum; biofertilizers; soybean; nodulation; crop yield
Categories
Funding
- Yibin University, Yibin, Sichuan province, China
- Apex Biotechnology Laboratory, Apex Holdings Ltd., Bangladesh
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The study showed that using PVP and Na-alginate as substrates can improve the survival rate and storage time of Bradyrhizobium japonicum strains, thereby significantly increasing the nodule number and yield of soybean in field experiments.
Nontoxic polymers PVP and Na-alginate may provide a favorable environment for the survival of bacteria. Therefore, PVP and Na-alginate were added to a growth medium to develop a liquid inoculant of Bradyrhizobium japonicum strain. The strain was identified by 16S rDNA sequencing. The addition of PVP (1.8%) and Na-alginate (0.2%) in the medium promoted a better survival (1.93 x 10(9) cells mL(-1)) of B. japonicum strain compared to the control (3.50 x 10(2) cells mL(-1)) after 6 months of storage. The combination of PVP and Na-alginate ensured 1.53 x 10(7) cells mL(-1) up to 12 months of storage under ambient temperature (28 +/- 2 degrees C), whereas PVP (1.8%) or Na-alginate (0.2%) alone produced similar cell counts only up to 8 months and 6 months, respectively. Consecutive field experiments proved the efficacy of the liquid inoculant on nodulation and yield of soybean. The combination of PVP and Na-alginate-based inoculation of B. japonicum strain significantly increased the nodule number per plant, number of pods per plant, number of seeds per pod, seed yield, and yield per hectare (p <= 0.05). Thus, the combination of PVP- and Na-alginate-based inoculation of B. japonicum has great potential to popularize the organic cultivation of soybean.
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