Journal
FOOD SCIENCE & NUTRITION
Volume 11, Issue 2, Pages 613-626Publisher
WILEY
DOI: 10.1002/fsn3.2467
Keywords
antioxidant activity; extract; phenolic compound; optimization
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This study assessed the optimization of phenol compound extraction from Mentha piperita using ultrasonic waves with response surface methodology. The independent variables (time, temperature, and ethanol concentration) significantly influenced the properties and antioxidant activity of the extract. The best extraction conditions were found to be 50 minutes at 65 degrees C with a 59.6% v/v ethanol/water solution.
In this study, optimization of the extraction of phenol compounds from Mentha piperita using ultrasonic waves with response surface methodology (RSM) was assessed. In this regard, a central composite design with three independent variables of time (5, 27.5, and 50 min), temperature (25, 45, and 65 degrees C), and concentrations of ethanol in the water-ethanol solution (0%, 50%, and 100%) was used. Besides, the antioxidant activity tests (DPPH radical scavenging assay, ferric reducing antioxidant power [FRAP], and oxidative stability indexes [OSI]) were examined. Significant effects of independent variables on the extraction of phenol compound, DPPH radical scavenging power, and OSI of M. piperita extract, with the regression coefficients of 0.89, 0.92, and 0.94, respectively, were noted. However, no significant difference in terms of the FRAP among different treatments was noted. Also, the best antioxidant activity of M. piperita was obtained by using the ultrasonic wave for 50 min at 65 degrees C and 59.6% v/v ethanol/water solution. While the findings of RSM confirmed the experimental results, due to the favored properties of M. piperita extract by the proposed method, further research to investigate possible applications in the food industry is recommended.
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