Seasonal Effect on Developmental Competence, Oxidative Status and Tubulin Assessment of Prepubertal Ovine Oocyte

Simple Summary Oocytes obtained from the ovaries of slaughtered prepubertal ewes can be incorporated into an in vitro embryo production system. The collection of this material is possible at two different times of the year, spring and autumn. The first period is linked to the natural reproductive cycle of the sheep. The second, on the other hand, is linked to the manipulation of the reproductive seasonality which allows the oestrus cycle to be controlled artificially. The analysis highlighted that the collection season influences oocytes quality from prepubertal donors in terms of improved energetic and oxidative status, microtubular organization, and developmental competence in oocytes recovered in spring. Data obtained underline that oocytes seem to be genetically and evolutionarily programmed to give their best in spring, this being the most favorable period for newborns. The reproductive seasonality of domestic animals is often manipulated in order to have more reproductive periods for commercial purposes related to the production of milk and meat. It is scientifically proven that such an alteration of the reproductive activity in sheep entails a deterioration in oocyte quality, leading to an inability to generate embryos. Since oocytes obtained from prepubertal ewes can be incorporated into an in vitro embryo production system and considering that their quality is crucial to the success of in vitro procedures, the aim of this work was to investigate the effect of seasons on the quality of prepubertal ovine oocytes collected in autumn and spring. Ovaries were collected from a local slaughterhouse from 30-40-day-old suckling lambs during both seasons. Following 24 h of in vitro maturation, oocytes developmental competence, reactive oxygen species (ROS) intracellular levels, and mitochondrial activity were evaluated, and a tubulin assessment was performed. The results on embryo production, as a percentage of first divisions and number of blastocysts obtained, were significantly higher in oocytes collected in the spring. Mitochondrial activity in oocytes was higher, and ROS production significantly lower, in spring than in autumn. Tubulin PTMs (tyrosinated and acetylated alpha-tubulin) showed a higher immunoreactivity in oocytes collected in spring compared with autumn sampling. Our data showed that seasons may affect the developmental competence, energetic status, and tubulin assessment of oocytes recovered from prepubertal ewes. Therefore, special care should be taken when choosing the period of the year for prepuberal ovine oocytes collection aimed at in vitro embryo reproduction programs.

Automated summary

The study found that oocytes collected in spring from prepubertal ewes have higher developmental competence and vitality in in vitro embryo production, with lower production of reactive oxygen species. The research indicates that the season of collection can affect the developmental competence, energetic status, and tubulin assessment of oocytes, emphasizing the importance of considering the season when collecting prepubertal ovine oocytes for in vitro embryo reproduction programs.