Beneficial Influence of Soybean Lecithin Nanoparticles on Rooster Frozen-Thawed Semen Quality and Fertility

Simple Summary Soy lecithin (SL) can be used in to prevent spermatozoa cryodamage during cryopreservation by mitigating the efflux of cholesterol or phospholipids, thus reducing the formation of intracellular ice crystals. SL nanoparticles (nano-SL) have a smaller particle size and higher solubilizing capacity as compared with those that have not undergone nanotreatment. Thus, they allow for a better interaction or coating of sperm, decreasing cold shock injury during freezing-thawing processes. The objective of this study was to determine the optimal concentration of nano-SL. In order to achieve this, we assessed the quality of frozen-thawed semen in vitro and in vivo. We found that a nano-SL dosage of 1.0% in the semen extender had an affirmative influence on post-thawing quality in roosters, improving various parameters related to sperm motion, protecting the membrane and acrosome integrities, increasing mitochondrial activity and antioxidant capacity, and reducing the oxidative stress caused by the cryopreservation process. Moreover, enrichment of 1.0% nano-SL in the semen extender improved the fertilizing capacity of rooster sperm after artificial insemination. The present study aimed to investigate the impact of different concentrations (0%, 0.5%, 1.0%, 1.5%, and 2.0%) of nano-soybean lecithin (SL) in the extender on sperm quality, sperm motion characteristics, and fertility outcomes of post-thawed rooster semen. Adult Ross broiler breeder roosters (n = 20) were subjected to semen collections twice a week for three weeks. At each collection, semen samples were pooled and allocated into five treatments corresponding to different nano-SL concentrations (control, SL0.5, SL1.0, SL1.5, and SL2.0). Sperm parameters, including motility (collected using a computer-assisted sperm analysis system), plasma membrane and acrosome integrities, and mitochondrial activity were assessed. Sperm malondialdehyde (MDA) and antioxidant activities (total antioxidant capacity (TAC); superoxide dismutase (SOD); glutathione peroxidase (GPx)) were evaluated. The fertility and hatchability obtained with frozen-thawed rooster semen supplemented with the optimum nano-SL concentration were assessed after artificial insemination. The results showed that the addition of 1% nano-SL into the extender led to a higher semen motility in roosters, improved plasma membrane and acrosome integrities, and higher mitochondrial activity of post-thawed rooster semen in comparison to controls (p < 0.05). The MDA levels in the SL0.5 and SL1.0 groups were lower than the other groups (p < 0.05). TAC activities in SL0.5, SL1.0, and SL1.5 groups were significantly higher than those in the other groups (p < 0.05). It was observed that the concentration of SOD was higher in the SL1.0 group than in the other groups (p < 0.05). The activity of GPx was not influenced in any of the cases (p > 0.05). Moreover, the percentages of fertility and hatchability in the SL1.0 group were higher (56.36% and 58.06%) than those in the control group (42.72% and 40.43%). In summary, the addition of nano-SL to the extenders enhanced the post-thawed semen quality and fertility of roosters by reducing the level of oxidative stress. The optimum nano-SL concentration was 1.0%. These results may be beneficial for improving the efficacy of semen cryopreservation procedures in poultry breeding.

Automated summary

Soy lecithin (SL) and its nanoparticles (nano-SL) can effectively prevent spermatozoa cryodamage during cryopreservation, with a concentration of 1.0% nano-SL showing the most positive impact on post-thawing semen quality and fertility in roosters. The addition of nano-SL reduced oxidative stress levels, improved sperm motility and mitochondrial activity, and enhanced fertility and hatchability in rooster semen after artificial insemination.