Impact of Diets Including Agro-Industrial By-Products on the Fatty Acid and Sterol Profiles of Larvae Biomass from Ephestia kuehniella, Tenebrio molitor and Hermetia illucens

Simple Summary Insects are a promising source of lipids. Their fatty acid compositions can vary as a function of diet composition, rearing conditions and developmental stage. In the present study, different agro-industrial by-products were used to feed the insects. Then, the fatty acids and sterols were determined. Notably, these profiles were assessed for the first time for E. kuehniella. According to our results, fatty acid profiles showed differences depending on diet composition, but mostly depended on species. Sterols varied significantly as a function of diet composition and species, showing low cholesterol and high campesterol and beta-sitosterol levels in H. illucens, and high cholesterol and low campesterol contents in T. molitor and E. kuehniella. These results suggest that insects are an interesting alternative source of fat for humans and animals, which might promote the use of insects for circular economy practices. Rearing insects on agro-industrial by-products is a sustainable strategy for the circular economy while producing valuable products for feed and foods. In this context, this study investigated the impact of larvae diet containing agro-industrial by-products on the contents of fatty acids and sterols of Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae), Tenebrio molitor (L.) (Coleoptera: Tenebrionidae), and Hermetia illucens (L.) (Diptera: Stratiomyidae). For each insect, selected diets were formulated using single or combined agro-industrial by-products (i.e., apricot, brewer's spent grain and yeast, and feed mill) and compared to a control diet. Fatty acid profiles showed differences depending on diet composition, but mostly depended on species: H. illucens was characterized by the abundance of C12:0, C16:0 and C18:2, whereas C:16, C18:1(n-9c), and C18:2(n-6c) were predominant in T. molitor and E. kuehniella. Sterols significantly varied as a function of diet composition and species. H. illucens showed low cholesterol levels and high campesterol and beta sitosterol levels (0.031, 0.554 and 1.035 mg/g, respectively), whereas T. molitor and E. kuehniella had high cholesterol and low campesterol contents (1.037 and 0.078 g/kg, respectively, for T. molitor; 0.873 and 0.132 g/kg, respectively, for E. kuehniella).

Automated summary

Insects are a potential source of lipids, with fatty acid compositions and sterols varying depending on diet composition and species. H. illucens has low cholesterol but high campesterol and beta-sitosterol levels, while T. molitor and E. kuehniella have high cholesterol and low campesterol contents. Rearing insects on agro-industrial by-products can be a sustainable strategy for the circular economy, providing valuable fat sources for humans and animals.