Relationships Between WUT (Body Weight, Urine Color, and Thirst Level) Criteria and Urine Indices of Hydration Status

Background: A Venn diagram consisting of percentage body mass loss, urine color, and thirst perception (weight, urine, thirst [WUT]) has been suggested as a practical method to assess hydration status. However, no study to date has examined relationships between WUT and urine hydration indices. Thus, the purpose of this study was to investigate relationships between urine specific gravity, urine osmolality, and the WUT criteria. Hypothesis: Urine specific gravity and urine osmolality indicate hypohydration when the WUT criteria demonstrate hypohydration (>= 2 markers). Study Design: Laboratory cohort study. Methods: A total of 22 women (mean +/- SD; age, 20 +/- 1 years; mass, 65.4 +/- 12.6 kg) and 21 men (age, 21 +/- 1 years; body mass, 78.7 +/- 14.6 kg) participated in this study. First morning body mass, urine color, urine specific gravity, urine osmolality, and thirst level were collected for 10 consecutive days in a free-living situation. Body mass loss >1%, urine color >5, and thirst level >= 5 were used as the dehydration thresholds. The number of markers that indicated dehydration levels were counted and categorized into either 3, 2, 1, or 0 WUT markers that indicated dehydration. One-way analysis of variance with Tukey pairwise comparisons was used to assess the differences in urine specific gravity and urine osmolality between the different number of WUT markers. Results: Urine specific gravity in 3 WUT markers (mean +/- SD [effect size], 1.021 +/- 0.007 [0.57]; P = 0.025) and 2 WUT markers (1.019 +/- 0.010 [0.31]; P = 0.026) was significantly higher than 1 WUT marker (1.016 +/- 0.009). Urine mosmolality in 2 WUT markers (705 +/- 253 mOsmol [0.43]; P = 0.018) was significantly higher than 1 WUT (597 +/- 253 mOsmol). Meeting at least 2 WUT markers resulted in sensitivities of 0.652 (2 WUT criteria met) and 0.933 (3 WUT criteria met) to detect urine osmolality >700 mOsmol. Conclusion: These results suggest that when 3 WUT markers are met, urine specific gravity and urine osmolality were greater than euhydration cutoff points. The WUT criterion is a useful tool to use in field settings to assess hydration status when first morning urine sample was used.

Automated summary

This study investigated the relationships between urine specific gravity, urine osmolality, and the WUT criteria, showing that when 3 WUT markers are met, urine specific gravity and urine osmolality were higher than euhydration cutoff points.