Journal
PHARMACEUTICS
Volume 13, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/pharmaceutics13081127
Keywords
experimental colitis; human uterine cervical stem cells; mucoadhesive and thermosensitive hydrogel; inflammation; conditioned medium delivery
Categories
Funding
- FEDER/Ministerio de Ciencia, Innovacion y Universidades [AEI/PGC2018-100776-B-100]
- Conselleria de Cultura, Educacion e Ordenacion Universitaria [ED431B 2021/04]
- European Union (ERDF/ESF, Investing in your future) [PI20/01122]
- 'Fundacion para la Investigacion con Celulas Madre Uterinas' (FICEMU)
- Xunta de Galicia [ED431C 2020/17-FEDER, AEMAT ED431E 2018/08]
- Instituto de Salud Carlos III
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This study found that using a hydrogel loaded with conditioned medium from human uterine cervical stem cells can effectively alleviate symptoms of colitis in mice.
Inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), is increasingly prevalent and current therapies are not completely effective. Mesenchymal stem cells are emerging as a promising therapeutic option. Here, the effect of local hydrogel application loaded with conditioned medium (CM) from human uterine cervical stem cells (hUCESC-CM) in an experimental acute colitis mice model has been evaluated. Colitis induction was carried out in C57BL/6 mice by dissolving dextran sulfate sodium (DSS) in drinking water for nine days. Ulcers were treated by rectal administration of either mesalazine (as positive control) or a mucoadhesive and thermosensitive hydrogel loaded with hUCESC-CM (H-hUCESC-CM). Body weight changes, colon length, and histopathological analysis were evaluated. In addition, pro-inflammatory TNF-alpha, IL-6, and IFN-gamma mRNA levels were measured by qPCR. Treatment with H-hUCESC-CM inhibited body weight loss and colon shortening and induced a significant decrease in colon mucosa degeneration, as well as TNF-alpha, IFN-gamma, and IL-6 mRNA levels. Results indicate that H-hUCESC-CM effectively alleviated DSS-induced colitis in mice, suggesting that H-hUCESC-CM may represent an attractive cell-free therapy for local treatment of IBD.
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