Journal
FRONTIERS IN ONCOLOGY
Volume 11, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fonc.2021.694331
Keywords
extramedullary multiple myeloma; MIF; immunohistochemistry staining; xenograft models; single-cell sequencing
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Funding
- National Natural Science Foundation of China [81800207, 81870157, 82070219]
- Science and Technology Department of Sichuan Province [2018FZ0030, 2019YJ0028]
- Sichuan University Faculty Start Fund
- Health Commission of Sichuan Province [18PJ357]
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The study revealed lower MIF expression in extramedullary multiple myeloma (EMM) compared to intramedullary multiple myeloma (IMM), with MIFHigh cells in IMM showing higher proliferation and MIFLow cells in EMM being more quiescent and enriched in ribosomal protein genes. In vivo differential regulation of MIF expression in MM was identified, suggesting a potential pathogenic role of MIF in the extramedullary spread of disease.
Macrophage migration inhibitory factor (MIF) has been shown to promote disease progression in many malignancies, including multiple myeloma (MM). We previously reported that MIF regulates MM bone marrow homing and knockdown of MIF favors the extramedullary myeloma formation in mice. Here, based on MIF immunostaining of myeloma cells in paired intramedullary and extramedullary biopsies from 17 patients, we found lower MIF intensity in extramedullary MM (EMM) versus intramedullary MM (IMM). Flow cytometry and histology analysis in xenograft models showed a portion of inoculated human MM cells lost their MIF expression (MIFLow) in vivo. Of note, IMM had dominantly MIFHigh cells, while EMM showed a significantly increased ratio of MIFLow cells. Furthermore, we harvested the extramedullary human MM cells from a mouse and generated single-cell transcriptomic data. The developmental trajectories of MM cells from the MIFHigh to MIFLow state were indicated. The MIFHigh cells featured higher proliferation. The MIFLow ones were more quiescent and harbored abundant ribosomal protein genes. Our findings identified in vivo differential regulation of MIF expression in MM and suggested a potential pathogenic role of MIF in the extramedullary spread of disease.
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