Journal
JOURNAL OF CLINICAL MEDICINE
Volume 10, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/jcm10122647
Keywords
genotyping; preeclampsia; MMP-9; MMP-2; SNPs
Categories
Funding
- Ministry of Higher Education and Scientific Research Tunisia
- Faculty of Pharmacy of Monastir, University of Monastir (Tunisia)
- CIHR operating grant [PJT-169020]
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This study found an increased frequency of heterozygous MMP-9 -1562 C/T genotype carriers in women with preeclampsia compared to healthy controls, suggesting it could be a genetic risk factor for preeclampsia in Tunisian women. However, there was no significant difference in the frequency distribution of the allele and genotype of MMP-2 -735 C/T polymorphism between healthy pregnant women and those with preeclampsia.
The abnormal production of matrix metalloproteinases (MMPs), especially MMP-9 and MMP-2, plays a pivotal role in hypertensive disorders of pregnancy, and as such, can influence the development of preeclampsia. These alterations may result from functional genetic polymorphisms in the promoter region of MMP-9 and MMP-2 genes, which modify MMP-9 and MMP-2 expression. We investigated the association of MMP-9 polymorphism rs3918242 (-1562 C>T) and MMP-2 polymorphism rs2285053 (-735 C>T) with the risk of preeclampsia. This case-control study was conducted on 345 women with preeclampsia and 281 age-matched women with normal pregnancies from Tunisian hospitals. Genomic DNA was extracted from whole blood collected at delivery. Genotypes for -1562 C>T and -735 C>T polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). An increased frequency of heterozygous MMP-9 -1562 C/T genotype carriers was observed in women with preeclampsia compared to healthy controls (p = 0.03). In contrast, the MMP-2 -735 C>T polymorphism was not significantly different regarding frequency distribution of the allele and genotype between healthy pregnant women and women with preeclampsia. Our study suggests that the MMP-9 -1562 C/T variant, associated with high MMP-9 production, could be a genetic risk factor for preeclampsia in Tunisian women.
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