Journal
SCIENCE ADVANCES
Volume 7, Issue 26, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abg4901
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Funding
- National Science Foundation [CBET2029361]
- National Cancer Institute [P30-CA014236, R01-CA248491, UH3-CA211232]
- Department of Defense United States Special Operations Command [W81XWH-16-C-0219]
- Defence Academy of the United Kingdom [ACC6010469]
- Combat Casualty Care Research Program (JPC-6) [W81XWH-17-2-0045]
- NHLBI [K08HL130557]
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The microfluidic point-of-care test can profile antibody responses against multiple SARS-CoV-2 antigens and detect the prognostic biomarker IP-10, making it essential for COVID-19 management. Its high sensitivity, specificity, and diagnostic and tracking capabilities for severe patients, along with the ability to be globally deployed, make it a valuable tool in combating COVID-19.
Highly sensitive, specific, and point-of-care (POC) serological assays are an essential tool to manage coronavirus disease 2019 (COVID-19). Here, we report on a microfluidic POC test that can profile the antibody response against multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens-spike S1 (S1), nucleocapsid (N), and the receptor binding domain (RBD)-simultaneously from 60.l of blood, plasma, or serum. We assessed the levels of antibodies in plasma samples from 31 individuals (with longitudinal sampling) with severe COVID-19, 41 healthy individuals, and 18 individuals with seasonal coronavirus infections. This POC assay achieved high sensitivity and specificity, tracked seroconversion, and showed good concordance with a live virus microneutralization assay. We can also detect a prognostic biomarker of severity, IP-10 (interferon-gamma-induced protein 10), on the same chip. Because our test requires minimal user intervention and is read by a handheld detector, it can be globally deployed to combat COVID-19.
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