Journal
SCIENCE ADVANCES
Volume 7, Issue 25, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abf0797
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Funding
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy CIBSS, EXC-2189 [390939984]
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Excellence Initiative of the German Federal and State Governments [EXC-294, GSC-4]
- Ministry for Science, Research, and Arts of the State of Baden-Wurttemberg
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The study developed an OptoAAV system that transfers genetic information into target cells upon red light illumination, enabling gene transfer with single-cell resolution, compatible with various cell lines and primary cells, and allowing spatially resolved transduction with different transgenes through sequential application of multiple OptoAAVs. This approach is likely extendable to other classes of viral vectors, facilitating advances in basic and applied genetic research.
Methodologies for the controlled delivery of genetic information into target cells are of utmost importance for genetic engineering in both fundamental and applied research. However, available methods for efficient gene transfer into user-selected or even single cells suffer from low throughput, the need for complicated equipment, high invasiveness, or side effects by off-target viral uptake. Here, we engineer an adeno-associated viral (AAV) vector system that transfers genetic information into native target cells upon illumination with cell-compatible red light. This OptoAAV system allows adjustable and spatially resolved gene transfer down to single-cell resolution and is compatible with different cell lines and primary cells. Moreover, the sequential application of multiple OptoAAVs enables spatially resolved transduction with different transgenes. The approach presented is likely extendable to other classes of viral vectors and is expected to foster advances in basic and applied genetic research.
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