Journal
ACS SENSORS
Volume 6, Issue 9, Pages 3468-3476Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.1c01437
Keywords
SARS-CoV-2 spike (S1) protein; biochip; coronavirus; double-interdigitated capacitive biosensor; point-of-care; high sensitivity and good linearity; diagnostic applications
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2018R1A6A1A03025242, 2018R1D1A1A09083353]
- Korea Ministry of Environment (MOE) Graduate School
- Kwangwoon University
Ask authors/readers for more resources
This research demonstrates the successful detection of SARS-CoV-2 S1 proteins using a functionalized GrO-glazed DIDC biosensing platform, showing enhanced selectivity and rapid response. The platform exhibits good sensitivity, a wide detection range, low limit of detection, good linearity, and high selectivity against antigens.
This research reveals the promising functionalization of graphene oxide (GrO)-glazed double-interdigitated capacitive (DIDC) biosensing platform to detect severe acute respiratory syndrome coronavirus (SARS-CoV-2) spike (S1) proteins with enhanced selectivity and rapid response. The DIDC bioactive surface consisting of Pt/Ti featured SiO2 substrate was fabricated using GrO/EDC-NHS/anti-SARS-CoV-2 antibodies (Abs) which is having layer-by-layer interface self-assembly chemistry method. This electroactive immune-sensing platform exhibits reproducibility and sensitivity with reference to the SI protein of SARS-CoV-2. The outcomes of analytical studies confirm that GrO provided a desired engineered surface for Abs immobilization and amplified capacitance to achieve a wide detection range (1.0 mg/mL to 1.0 fg/mL), low limit of detection (1 fg/mL) within 3 s of response time, good linearity (18.56 nF/g), and a high sensitivity of 1.0 fg/mL. Importantly, the unique biochip was selective against blood-borne antigens and standby for 10 days at 5 degrees C. Our developed DIDC-based SARS-CoV-2 biosensor is suitable for point-of-care (POC) diagnostic applications due to portability and scaling-up ability. In addition, this sensing platform can be modified for the early diagnosis of severe viral infections using real samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available