Journal
FOOD BIOSCIENCE
Volume 42, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.fbio.2021.101161
Keywords
Collagen peptides; Sheep skin; Dipeptidyl peptidase IV inhibitory; LC-MS/MS identification; Gastrointestinal digestion stability
Categories
Funding
- National Natural Science Foundation of China [31871846, 31801589]
- Natural Science Foundation of Jiangsu Province [BK20180615]
- Fundamental Research Funds for the Central Universities [JUSRP12005]
- 111 project [B07029]
- national first-class discipline program of Food Science and Technology [JUFSTR20180204]
- program of Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, China
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Collagen peptides from sheep skin with DPP-IV inhibitory activity were identified, showing potential as functional components in diabetic diet. These peptides were able to maintain inhibitory activity post-digestion, indicating their resilience and importance in controlling DPP-IV levels. Molecular docking studies revealed the mechanisms behind their DPP-IV inhibitory activity.
Collagen is a potential precursor protein for Dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides because of its high content of Pro. Collagen peptides with DPP-IV inhibitory activity were prepared and identified from sheep skin. The peptide sequences with the highest potential for DPP-IV inhibition were recognized by nano LS-MS/MS and in silico analysis. DPP-IV inhibitory activity of collagen peptides usually decreased after digestion, therefore the effect of peptides digestion on DPP-IV inhibitory activity were studied. Four DPP-IV inhibitory peptides resistant to digestive enzymes or having the possibility of generating new DPP-IV inhibitory peptides after digestion were synthesized. The activity of synthetic peptides before and after digestion were also studied. GPAGPIGPV was resistant to digestion and GPAGPOGFPG discharged DPP-IV inhibitory peptides in silico digestion, suggesting that they can maintain high DPP-IV inhibitory activity after digestion. Binding sites of the peptides with DPP-IV were studied by molecular docking, indicating the mechanism of the peptide having DPP IV inhibitory activity. The results indicated that DPP-IV-inhibitory peptides identified from sheep skin collagen may be functional components in diabetic diet.
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