Journal
HORTICULTURE RESEARCH
Volume 8, Issue 1, Pages -Publisher
OXFORD UNIV PRESS INC
DOI: 10.1038/s41438-021-00631-x
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [31800563, 31670682]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [2020YQ003]
- Key Research and Development Program of Zhejiang Province [2021C02001]
- Young Elite Scientists Sponsorship Program by the China Academy of Space Technology (CAST) [2018QNRC001]
Ask authors/readers for more resources
The study highlights the role of melatonin in regulating plant hormone biosynthesis and metabolism, while also showing that abscisic acid and ethylene can regulate melatonin biosynthesis. This provides insights into novel regulatory mechanisms and interactions between different plant hormones in plants.
Studies have shown that melatonin regulates the expression of various elements in the biosynthesis and catabolism of plant hormones. In contrast, the effects of these different plant hormones on the biosynthesis and metabolism of melatonin and their underlying molecular mechanisms are still unclear. In this study, the melatonin biosynthesis pathway was proposed from constructed metabolomic and transcriptomic libraries from hickory (Carya cathayensis Sarg.) nuts. The candidate pathway genes were further identified by phylogenetic analysis, amino-acid sequence alignment, and subcellular localization. Notably, most of the transcription factor-related genes coexpressed with melatonin pathway genes were hormone-responsive genes. Furthermore, dual-luciferase and yeast one-hybrid assays revealed that CcEIN3 (response to ethylene) and CcAZF2 (response to abscisic acid) could activate melatonin biosynthesis pathway genes, a tryptophan decarboxylase coding gene (CcTDC1) and an N-acetylserotonin methyltransferase coding gene (CcASMT1), by directly binding to their promoters, respectively. Our results provide a molecular basis for the characterization of novel melatonin biosynthesis regulatory mechanisms and demonstrate for the first time that abscisic acid and ethylene can regulate melatonin biosynthesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available