Journal
FRONTIERS IN GENETICS
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2021.680457
Keywords
Capsicum annuum; CaNHX; phylogenetic tree; co-expression; abiotic stress
Categories
Funding
- China Postdoctoral Science Foundation [2017T100718]
- National Natural Science Foundation of China [31660575, 31860498]
- Guizhou Province Science and Technology Plan Program of China [[2019]2258, [2020]1Y088, [2019]2413]
- Government Special Funds for Guiding Local Science and Technology Development of China [20174003]
- Zunyi Innovative Talent Team Training Project of China [201904]
- Zunyi Excellent Young Scientific and Technological Innovative Talents Training Project [201806]
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This study systematically identified and analyzed the CaNHX gene family in pepper using bioinformatics methods, providing insights into gene structure, functional domains, conserved motifs, and expression characteristics under salt stress. The results indicate that CaNHX genes play a crucial role in stress resistance and growth development in pepper.
The Na+/H+ exchangers (NHXs) are a class of transporters involved in ion balance during plant growth and abiotic stress. We performed systematic bioinformatic identification and expression-characteristic analysis of CaNHX genes in pepper to provide a theoretical basis for pepper breeding and practical production. At the whole-genome level, the members of the CaNHX gene family of cultivated and wild pepper were systematically identified using bioinformatics methods. Sequence alignment and phylogenetic tree construction were performed using MEGA X software, and the gene functional domain, conserved motif, and gene structure were analyzed and visualized. At the same time, the co-expression network of CaNHX genes was analyzed, and salt-stress analysis and fluorescence quantitative verification of the Zunla-1 cultivar under stress conditions were performed. A total of 9 CaNHX genes were identified, which have typical functional domains of the Na+/H+ exchanger gene. The physical and chemical properties of the protein showed that the protein was hydrophilic, with a size of 503-1146 amino acids. Analysis of the gene structure showed that Chr08 was the most localized chromosome, with 8-24 exons. Cis-acting element analysis showed that it mainly contains cis-acting elements such as light response, salicylic acid response, defense, and stress response. Transcriptom and co-expression network analysis showed that under stress, the co-expressed genes of CaNHX genes in roots and leaves were more obvious than those in the control group, including ABA, IAA, and salt. The transcriptome and co-expression were verified by qRT-PCR. In this study, the CaNHX genes were identified at the genome level of pepper, which provides a theoretical foundation for improving the stress resistance, production, development, and utilization of pepper in genetic breeding.
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