Journal
ACS CENTRAL SCIENCE
Volume 7, Issue 8, Pages 1400-1407Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscentsci.1c00645
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Funding
- National Key R&D Program of China [2018YFA0902600]
- NSFC [21804091, 21904086, 21804088]
- Shanghai Education Development Foundation
- Shanghai Municipal Education Commission [18SG16]
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support [20171913]
- Innovative Research Team of High Level Local Universities in Shanghai [SSMUZLCX20180701]
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Researches developed a method based on DNA origami nanostructures to reconstruct soma-soma synapse-like junctions, demonstrating the maintenance of intercellular communication. This strategy provides a versatile platform for studying synaptic communications.
Cell-cell communications exhibit distinct physiological functions in immune responses and neurotransmitter signaling. Nevertheless, the ability to reconstruct a soma-soma synapse-like junction for probing intercellular communications remains difficult. In this work, we develop a DNA origami nanostructure-based method for establishing cell conjugation, which consequently facilitates the reconstruction of a soma-soma synapse-like junction. We demonstrate that intercellular communications including small molecule and membrane vesicle exchange between cells are maintained in the artificially designed synapse-like junction. By inserting the carbon fiber nanometric electrodes into the soma-soma synapse-like junction, we accomplish the real-time monitoring of individual vesicular exocytotic events and obtain the information on vesicular exocytosis kinetics via analyzing the parameters of current spikes. This strategy provides a versatile platform to study synaptic communications.
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