Journal
ACS CENTRAL SCIENCE
Volume 7, Issue 8, Pages 1419-1426Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscentsci.1c00670
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Funding
- NIH [1R01GM131372, 1R35GM134963, R01GM118486, 1T32EB019941, P30DK045735]
- Wellcome Trust [203285/B/16/Z]
- Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy [DE-AC02-05CH11231]
- Wellcome Trust [203285/B/16/Z] Funding Source: Wellcome Trust
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New photostable, emission-orthogonal fluorophores such as Yale(676sb) are needed for multicolor, live-cell, single-molecule localization microscopy. With an exceptional quantum yield of 0.59, Yale(676sb) enables simultaneous two-color SMLM of intracellular organelles without the need for toxic additives.
New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale(676sb), a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale(676sb) enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
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