Journal
INFLAMMOPHARMACOLOGY
Volume 29, Issue 4, Pages 1241-1253Publisher
SPRINGER BASEL AG
DOI: 10.1007/s10787-021-00821-y
Keywords
Antihyperalgesia; Allodynia; Chronic constriction injury; Nitric oxide; Cytokines; P; macrocarpus
Categories
Funding
- Centre for Science and Technology of the Non Aligned and Other Developing Countries (NAMS TCentre)
Ask authors/readers for more resources
The study found that stem bark extracts of P. macrocarpus have a potent anti-hypernociceptive effect on CCI neuropathic pain, possibly achieved through inhibition of the nitric oxide pathway and reduction of NF-kappa B and TNF-alpha gene expression in the brain.
Petersianthus macrocarpus (Lecythidaceae) stem bark is traditionally used in West and Central Africa for the treatment of boils and pain. The present study examined the chemical composition of the aqueous and methanolic stem bark extracts of P. macrocarpus by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) . Their antinociceptive effect was evaluated using chronic constriction injury (CCI)-induced neuropathic pain in a rat model. On the ninth day post-surgery, the pain perception (allodynia and hyperalgesia) of the animals was assessed after the administration of aqueous and methanolic extracts at the doses of 100 and 200 mg/kg. In addition, the effect of the extracts was evaluated on nitric oxide activity and on the expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, and NF-kappa B). The LC-ESI-MS analysis revealed the presence of ellagic acid as the major constituent in the methanol extract. Both extracts at the employed doses (100 and 200 mg/kg), significantly (p p < 0.001) reduced the spontaneous pain, tactile and cold allodynia, and mechanical hyperalgesia. The methanolic extract used at the dose of 200 mg/kg significantly reduced the nitric oxide level (p < 0.001) and the gene expression levels of NF-kappa B (p < 0.05) and TNF-alpha (p < 0.01) in the brain. These data may indicate that stem bark extracts of P. macrocarpus possess a potent anti-hypernociceptive effect on CCI neuropathic pain. The inhibition of the nitric oxide pathway as well as the reduction in NF-kappa B and TNF-alpha gene expression in the brain may at least partially contribute to this effect. The results further support the use of this plant by traditional healers in pain conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available