Journal
GEOMICROBIOLOGY JOURNAL
Volume 34, Issue 2, Pages 183-192Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/01490451.2016.1189015
Keywords
Arsenic; DGGE; EL-FAME; MicroResp; soil enzymes
Funding
- Italian Ministry of University and Research, Research Projects of National Interest PRIN [2010JBNLJ7-004]
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Microbial genetic, structural and functional diversity was assessed in response to arsenic (As) pollution in a former gold mine soil. Ester-linked fatty acid methyl ester (EL-FAME), quantitative PCR (qPCR), denaturating gradient gel electrophoresis (DGGE), enzyme activities and MicroResp techniques were used.Multivariate analysis showed that As bioavailability in soil was an important driver affecting microbial diversity. Microbial biomass assessed by EL-FAMEs and qPCR generally decreased under higher bioavailable As, as well as enzyme activities and C substrate utilization. Conversely, actinomycetes and fungal biomass increased along with total As content suggesting the selection of more resistant species.
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