Journal
FRONTIERS IN IMMUNOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.696148
Keywords
Bacillus subtilis; KMOS; Caco-2 cells; LPS; intestinal injury
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Funding
- Fundamental Research Funds for the Central Universities [2662019FW012]
- National Key R&D Program of China [2018YFD0500204]
- Science and Technology Program of Wuhan, China [2016020101010091]
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This study demonstrates that the combination of konjac mannan oligosaccharides (KMOS) and Bacillus subtilis (BS) has a synergistic repair effect on inflammatory and oxidative damage of Caco-2 cells, as well as alleviates LPS-induced acute intestinal injury in mice. Co-treatment with KMOS and BS enhances the activity and antioxidant capacity of Caco-2 cells, protects mouse liver and ileum from oxidative damage, and repairs tight junction and mucus barrier damage.
As the first line of defense against intestinal bacteria and toxins, intestinal epithelial cells are always exposed to bacteria or lipopolysaccharide (LPS), whereas pathogenic bacteria or LPS can cause intestinal epithelial cell damage. Previous studies have shown that konjac mannan oligosaccharides (KMOS) have a positive effect on maintaining intestinal integrity, and Bacillus subtilis (BS) can promote the barrier effect of the intestine. However, it is still unknown whether KMOS and BS have a synergistic protective effect on the intestines. In this study, we used the LPS-induced Caco-2 cell injury model and mouse intestinal injury model to study the synergistic effects of KMOS and BS. Compared with KMOS or BS alone, co-treatment with KMOS and BS significantly enhanced the activity and antioxidant capacity of Caco-2 cell, protected mouse liver and ileum from LPS-induced oxidative damage, and repaired tight junction and mucus barrier damage by up-regulating the expression of Claudin-1, ZO-1 and MUC-2. Our results demonstrate that the combination of KMOS and BS has a synergistic repair effect on inflammatory and oxidative damage of Caco-2 cells and aIIeviates LPS-induced acute intestinal injury in mice.
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