Journal
FRONTIERS IN IMMUNOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.702359
Keywords
Mycobacterium tuberculosis; proteomics; NF-KB signalling; PE; PPE; macrophage
Categories
Funding
- NRF under the NRF-VU Desmond Tutu Doctoral training program
- Harry Crossley Foundation
- South African National Research Foundation Department of Science and Technology Innovation Postdoctoral Fellowship [SFP13071721852]
- South African Research Chairs Initiative of the Department of Science and Technology
- National Research Foundation (NRF) of South Africa [UID 86539]
- SA MRC Centre for TB Research
- DST/NRF Centre of Excellence for Biomedical Tuberculosis Research
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Study found that PPE38-deficient M. tuberculosis resulted in decreased pro-inflammatory response in macrophages compared to wild type bacteria. This phenomenon was associated with the activation of the RelB/p50 pathway, indicating a molecular mechanism by which PPE38 controls macrophage responses through NF-kB signaling.
It was previously shown that secretion of PE-PGRS and PPE-MPTR proteins is abolished in clinical M. tuberculosis isolates with a deletion in the ppe38-71 operon, which is associated with increased virulence. Here we investigate the proteins dependent on PPE38 for their secretion and their role in the innate immune response using temporal proteomics and protein turnover analysis in a macrophage infection model. A decreased pro-inflammatory response was observed in macrophages infected with PPE38-deficient M. tuberculosis CDC1551 as compared to wild type bacteria. We could show that dampening of the pro-inflammatory response is associated with activation of a RelB/p50 pathway, while the canonical inflammatory pathway is active during infection with wild type M. tuberculosis CDC1551. These results indicate a molecular mechanism by which M. tuberculosis PE/PPE proteins controlled by PPE38 have an effect on modulating macrophage responses through NF-kB signalling.
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