Journal
APPLIED SCIENCES-BASEL
Volume 11, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/app11167201
Keywords
Haematococcus pluvialis; germination; cultivation conditions; extraction; astaxanthin; downstream processing
Categories
Funding
- FEDERAL MINISTRY OF ECONOMIC AFFAIRS AND ENERGY (BMWi) [ZF4025031 SB8]
- FOUNDATION OF GERMAN BUSINESS (sdw)
- German Research Foundation (DFG)
- Technical University of Munich
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The microalgae Haematococcus pluvialis is used for biotechnological production of astaxanthin, with extraction achieved by germination of cyst cells instead of mechanical disruption, improving extraction efficiency.
The microalgae Haematococcus pluvialis is used for the biotechnological production of astaxanthin. The red carotenoid accumulates in the cytoplasm under unfavorable conditions. Astaxanthin synthesis is associated with the transformation of motile vegetative cells into non-motile cyst cells. In the industrial process, after harvesting, the cyst cells are mechanically disrupted, dried, and finally, astaxanthin is extracted with supercritical CO2. The germination of the cyst cells represents an interesting alternative, replacing the mechanical cyst cell wall disruption. When cyst cells are exposed to favorable growth conditions, germination of the cyst cells occurs and zoospores are released after a certain time. These zoospores show a much weaker cell matrix compared to cyst cells. In this study, germination under phototrophic, mixotrophic, and heterotrophic conditions was examined. Glucose was used as the carbon source for mixotrophic and heterotrophic germination. Applying heterotrophic conditions, up to 80% of the cells were in the zoospore stage 49 h after the start of germination, and extraction yields of up to 50% were achieved using the solvent ethyl acetate for the extraction of astaxanthin from the algal broth containing zoospores. An extraction yield of up to 64% could be achieved by doubling the nitrate concentration and combining mixotrophic and heterotrophic cultivation.
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