Journal
APPLIED SCIENCES-BASEL
Volume 11, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/app11178273
Keywords
Pentoxifylline (PTX); immunoprecipitation high-performance liquid chromatography (IP-HPLC); RAW 264; 7 cell; immunomodulation; osteoradionecrosis; COVID-19
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2021R1I1A1A01058939]
- National Research Foundation of Korea [2021R1I1A1A01058939] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The study showed that PTX plays an immunomodulatory role in RAW 264.7 cells by regulating various proteins, enhancing immunity and osteogenesis-related proteins, while decreasing proliferation, inflammation, and cellular apoptosis-related proteins expressions.
Pentoxifylline (PTX) is a methylxanthine derivative that has been developed as an immunomodulatory agent and an improvement of microcirculation. Osteoradionecrosis (ORN) is a serious complication of radiation therapy due to hypovascularity. Coronavirus disease 2019 (COVID-19) has spread globally. Symptoms for this disease include self-limiting respiratory tract illness to severe pneumonia and acute respiratory distress. In this study, the effects of PTX on RAW 264.7 cells were investigated to reveal the possibility of PTX as a therapeutic agent for ORN and COVID-19. To reveal PTX effects at the cellular level, protein expression profiles were analyzed in the PTX-treated RAW 264.7 cells by using immunoprecipitation high-performance liquid chromatography (IP-HPLC). PTX-treated RAW 264.7 cells showed increases in immunity- and osteogenesis-related proteins and concurrent decreases in proliferation-, matrix inflammation-, and cellular apoptosis-related proteins expressions. The IP-HPLC results indicate that PTX plays immunomodulatory roles in RAW 264.7 cells by regulating anti-inflammation-, proliferation-, immunity-, apoptosis-, and osteogenesis-related proteins. These results suggest that PTX may be used as supplement medications for ORN as well as for COVID-19.
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