4.5 Article

Production of R- and S-1,2-propanediol in engineered Lactococcus lactis

Journal

AMB EXPRESS
Volume 11, Issue 1, Pages -

Publisher

SPRINGER
DOI: 10.1186/s13568-021-01276-8

Keywords

Bioproduction; Fermentation; Lactococcus lactis; 1; 2-Propanediol

Funding

  1. Japan Science and Technology Agency-Mirai RD Program [JPMJMI17EC]

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This study demonstrates the production of R- and S-1,2-propanediol using engineered Lactococcus lactis for the first time. The addition of mannitol and gluconate in the culture medium enhances 1,2-propanediol production, and a pH-stat approach is used to achieve high concentration of S-1,2-propanediol.
1,2-propanediol (1,2-PDO) is a versatile chemical used in multiple manufacturing processes. To date, some engineered and non-engineered microbes, such as Escherichia coli, Lactobacillus buchneri, and Clostridium thermosaccharolyticum, have been used to produce 1,2-PDO. In this study, we demonstrated the production of R- and S-1,2-PDO using engineered Lactococcus lactis. The L- and D-lactic acid-producing L. lactis strains NZ9000 and AH1 were transformed with the plasmid pNZ8048-ppy harboring pct, pduP, and yahK genes for 1,2-PDO biosynthesis, resulting in L. lactis LL1 and LL2, respectively. These engineered L. lactis produced S- and R-1,2-PDO at concentrations of 0.69 and 0.50 g/L with 94.4 and 78.0% ee optical purities, respectively, from 1% glucose after 72 h of cultivation. Both 1% mannitol and 1% gluconate were added instead of glucose to the culture of L. lactis LL1 to supply NADH and NADPH to the 1,2-PDO production pathway, resulting in 75% enhancement of S-1,2-PDO production. Production of S-1,2-PDO from 5% mannitol and 5% gluconate was demonstrated using L. lactis LL1 with a pH-stat approach. This resulted in S-1,2-PDO production at a concentration of 1.88 g/L after 96 h of cultivation. To our knowledge, this is the first report on the production of R- and S-1,2-PDO using engineered lactic acid bacteria.

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