Journal
ADVANCED SCIENCE
Volume 8, Issue 21, Pages -Publisher
WILEY
DOI: 10.1002/advs.202102327
Keywords
cryomicroneedles; drug delivery; eye infection; ocular disease; predatory bacteria
Categories
Funding
- City University of Hong Kong [9610472]
- General Research Fund (GRF) from University Grant Committee of Hong Kong (UGC) Research Grant Council (RGC) [9042951, 9043133]
- NSFC/RGC Joint Research Scheme [N_CityU118/20, 9054032]
- Singapore Ministry of Health's National Medical Research Council under its Centre Grant Programme - Optimization of Core Platform Technologies for Ocular Research [NMRC/CG/M010/2017_SERI]
- SingHealth Foundation [SHF/FG663P/2017]
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The study introduced cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria, demonstrating their effectiveness in treating ocular bacterial infections and opening new avenues for the treatment of ocular surface disorders.
The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long-term storage studies, and they successfully impede the growth of gram-negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders.
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