Journal
ADVANCED SCIENCE
Volume 8, Issue 19, Pages -Publisher
WILEY
DOI: 10.1002/advs.202101902
Keywords
epicuticular wax; infrared; microscopy; plant physiology; reflectance
Categories
Funding
- Australian Government
- Curtin University Faculty of Science Engineering's Small Grants Program
- AINSE-PGRA top-up scholarship
- AINSE-EMCR Award
- Australian Research Council (ARC Future Fellowship) [FT190100017]
- Australian grains industry through the Grains Research and Development Corporation [CUR00023]
- ANSTO
- Australian Research Council [FT190100017] Funding Source: Australian Research Council
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The analysis of the epicuticular wax layer on plant leaves using FTIR reflectance spectroscopic imaging provides a non-destructive in situ method to investigate the distribution of wax in plants, offering insights into how plants respond to growth, disease, and environmental stressors.
Analysis of the epicuticular wax layer on the surface of plant leaves can provide a unique window into plant physiology and responses to environmental stimuli. Well-established analytical methodologies can quantify epicuticular wax composition, yet few methods are capable of imaging wax distribution in situ or in vivo. Here, the first report of Fourier transform infrared (FTIR) reflectance spectroscopic imaging as a non-destructive, in situ, method to investigate variation in epicuticular wax distribution at 25 mu m spatial resolution is presented. The authors demonstrate in vivo imaging of alterations in epicuticular waxes during leaf development and in situ imaging during plant disease or exposure to environmental stressors. It is envisaged that this new analytical capability will enable in vivo studies of plants to provide insights into how the physiology of plants and crops respond to environmental stresses such as disease, soil contamination, drought, soil acidity, and climate change.
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