The Mexican giant maize of Jala landrace harbour plant-growth-promoting rhizospheric and endophytic bacteria

The giant landrace of maize Jala is a native crop cultured in Nayarit and Jalisco States in the occident of Mexico. In this study, after screening 374 rhizospheric and endophytic bacteria isolated from rhizospheric soil, root, and seed tissues of maize Jala, a total of 16 bacterial strains were selected for their plant-growth-promoting potential and identified by 16S rRNA phylogenetic analysis. The isolates exhibited different combinations of phenotypic traits, including solubilisation of phosphate from hydroxyapatite, production of a broad spectrum of siderophores such as cobalt, iron, molybdenum, vanadium, or zinc (Co2+, Fe3+, Mo2 +, V5+, Zn2+), and nitrogen fixation capabilities, which were detected in both rhizospheric and endophytic strains. Additional traits such as production of 1-aminocyclopropane-1-carboxylate deaminase and a high-rate production of Indoleacetic Acid were exclusively detected on endophytic isolates. Among the selected strains, the rhizospheric Burkholderia sp., and Klebsiella variicola, and the endophytic Pseudomonas protegens significantly improved the growth of maize plants in greenhouse assays and controlled the infection against Fusarium sp. 50 on fresh maize cobs. These results present the first deep approach on handling autochthonous microorganisms from native maize with a potential biotechnological application in sustainable agriculture as biofertilizers or biopesticides.

Automated summary

This study isolated and identified 16 bacterial strains with plant-growth-promoting potential from maize Jala, showing promising use as biofertilizers or biopesticides in sustainable agriculture. Among the selected strains, Burkholderia sp., Klebsiella variicola, and Pseudomonas protegens significantly improved maize plant growth and controlled infection against Fusarium sp. 50.