Production and Recovery of Exo-polygalacturonase from Umbu (Spondias tuberosa) Residue

The objective of the present work was to produce exo-polygalacturonase by solid-state fermentation of the dehydrated peels of umbu (Spondias tuberosa) using Aspergillus niger CCT 0916 and then, using a biphasic aqueous system, recover the enzyme. The peels were dehydrated at 55 degrees C to obtain the dried residue and then used to produce the exo-polygalacturonase using Aspergillus niger CCT 0916 and solid-state fermentation. During the 72 h fermentation, the pH, reducing sugars, and the exo-polygalacturonase activity were tested. Then the extracted enzyme was recovered with a biphasic aqueous system composed of PEG/potassium phosphate. The umbu dried residue showed a maximum peak of enzymatic activity with values of 17.30 U/g after 44 h of fermentation. The enzyme extraction using the dried umbu residue yielded a maximum PGA (Activity polygalacturonase) of 32.59 U/g. The biphasic aqueous system composed of PEG8000 and potassium phosphate salts yielded the highest recovery of 97.14%.

Automated summary

This study aimed to produce exo-polygalacturonase through solid-state fermentation of dehydrated umbu peels using Aspergillus niger CCT 0916, followed by recovery of the enzyme using a biphasic aqueous system. The dried umbu peel residue showed the highest enzyme activity peak after 44 hours of fermentation, with the maximum recovery rate of 97.14% achieved using a biphasic aqueous system composed of PEG8000 and potassium phosphate salts.