Extracting and Physicochemical Properties of Carotenoprotein from Shrimp Processing Waste by Proteases-Mediated Hydrolysis

In this study, carotenoprotein from Pacific white shrimp (Litopenaeus vannamei) processing waste was extracted with the aid of alcalase (CP-A) and pepsin (CP-P) at various levels (0-4 units/100 g waste) for different times (0-240 min). Recovery of carotenoids and protein increased with 3 unit/g of enzyme and hydrolysis times until 180 min (p < 0.05). The extracted carotenoprotein by pepsin and alcalase consisted of 72.11-75.32% protein and carotenoids content was in the range of 330-530 mu g/g samples. The phenylalanine, lysine, methionine, and valine as essential amino acids were higher at CP-A and CP-P. The dominant non-essential amino acids in carotenoproteins were aspartic acid, glutamic acid, glycine, and alanine. It was rich in mono and polyunsaturated fatty acids. The CP-A showed higher content of docosahexaenoic acid and eicosapentaenoic acid (8.52 and 6.49%) than CP-P (5.55 and 5.49%). The saturated fatty acids were reduced after enzymatic hydrolysis and contents were higher in carotenoproteins. The extracted samples showed a significant amount of mineral contents. Sodium, phosphorus, magnesium, and potassium contents were found higher in CP-A. The lead and copper were reduced as a result of hydrolysis. Therefore, carotenoprotein from processing residue of pacific white shrimp could be used as the value-added nutritious enriching food or feed powder. [GRAPHICS] .

Automated summary

Carotenoprotein extracted from Pacific white shrimp processing waste using alcalase and pepsin showed high protein and carotenoid content, with essential amino acids and dominant non-essential amino acids. The extracted samples also contained high amounts of mono and polyunsaturated fatty acids, with differences in fatty acid compositions between alcalase and pepsin extracted samples. Mineral content was significant in the extracted samples, with higher levels of certain minerals in alcalase-extracted samples. The results suggest that carotenoprotein from processing residue of Pacific white shrimp can be used as a valuable and nutritious food or feed powder.