Journal
PEERJ
Volume 9, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.11707
Keywords
Maize; Chilling stress; Seed germination; Genome-wide association study; Candidate genes
Categories
Funding
- National Natural Science Foundation of China [31871637, 32072073]
- Sichuan Science, and Technology Program [2021JDTD0004, 2021YJ0476, 2019YJ0511]
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By investigating 300 inbred lines of maize, this study identified several important gene loci and candidate genes associated with seed germination under chilling stress. These findings contribute to a better understanding of the genetic and molecular mechanisms underlying chilling germination in maize.
As one of the major crops, maize (Zea mays L.) is mainly distributed in tropical and temperate regions. However, with the changes of the environments, chilling stress has become a significantly abiotic stress affecting seed germination and thus the reproductive and biomass accumulation of maize. Herein, we investigated five seed germination-related phenotypes among 300 inbred lines under low-temperature condition (10 degrees C). By combining 43,943 single nucleotide polymorphisms (SNPs), a total of 15 significant (P < 2.03 x 10(-6)) SNPs were identified to correlate with seed germination under cold stress based on the FarmCPU model in GWAS, among which three loci were repeatedly associated with multiple traits. Ten gene models were closely linked to these three variations, among which Zm00001d010454, Zm00001d010458, Zm00001d010459, and Zm00001d050021 were further verified by candidate gene association study and expression pattern analysis. Importantly, these candidate genes were previously reported to involve plant tolerance to chilling stress and other abiotic stress. Our findings contribute to the understanding of the genetic and molecular mechanisms underlying chilling germination in maize.
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