Journal
PEERJ
Volume 9, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.11484
Keywords
EpCAM; Dimerization; TACE; Proteolytic cleavage
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Funding
- Slovenian Research Agency [Z1-2637, J1-7119, P10140, P1-0207]
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EpCANI is a cell-surface protein utilized as a carcinoma marker, involved in nuclear signaling via RIP. Research has shown that the oligomeric state of EpCAM is a determinant in RIP, with the disulfide-stabilized dimer being more resistant to cleavage than the monomeric form.
The cell-surface protein EpCANI is a carcinoma marker utilized in diagnostics and prognostics, and a promising therapeutic target. It is involved in nuclear signaling via regulated intramembrane proteolysis (RIP). Many aspects of this process are not fully understood, including the events at the molecular level leading to the exposure of cleavage sites, buried at the dimerization interface. To investigate the effect of dimer stability on cleavage susceptibility we prepared two mutants of human EpCAM ectodomain: a monomeric form, and a disulfide-stabilized dimeric form. We show that the disulfide-stabilized dimer is resistant to tumor necrosis factora -converting enzyme (TACE) cleavage, while the monomeric form is more susceptible than the predominantly dimeric wild type. This provides experimental evidence that the oligomeric state of EpCAM is a determinant in RIP and demonstrates the usefulness of the oligomeric state-specific mutants in investigations of EpCAM biological function.
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