Journal
NANOMATERIALS
Volume 11, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/nano11061496
Keywords
lateral flow assay (LFA) strip; surface-enhanced Raman scattering (SERS); point-of-care testing (POCT); inflammatory biomarkers; core-shell nanoparticles; portable cloud Raman spectrometer
Categories
Funding
- Shenzhen Science and Technology Program [JSGG2019 1129105842769, KQTD20170809110344233]
- Shenzhen Bay Laboratory [SZBL201906281005]
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In this study, a lateral flow assay (LFA) strip based on surface-enhanced Raman scattering (SERS) nanotags was proposed for the simultaneous and quantitative detection of dual infection biomarkers SAA and CRP. A smartphone-based portable Raman spectrometer could accurately detect Raman signals at concentrations as low as 10 ng/mL, showing great potential for rapid on-site diagnosis of infections.
Inflammatory biomarkers are closely related to infectious diseases. However, traditional clinical tests of laboratory inspection are unable to achieve rapid and accurate detection of these biomarkers on-site due to shortcomings such as complex experimental operation, expensive equipment, and long test time. Herein, we proposed a lateral flow assay (LFA) strip based on surface-enhanced Raman scattering (SERS) nanotags (SERS-LFA strips) for the simultaneous and quantitative detection of dual infection biomarkers, serum amyloid A (SAA) and C-reactive protein (CRP), respectively. In practice, mesoporous silica (mSiO(2))-coated Au nanoparticles (Au NPs) were used as the SERS substrate. Mercaptobenzoic acid (MBA) was embedded in the internal gap between Au NPs and the mSiO(2) shell to prepare Au-MBA@mSiO(2) NPs, onto which SAA and CRP antibodies were modified to prepare two Au-MBA@mSiO(2) SERS nanotags. The Raman intensities of the test and control lines were simultaneously identified for the qualitative detection of SAA and CRP, with limits of detection (LODs) as low as 0.1 and 0.05 ng/mL for SAA and CRP, respectively. Finally, aiming at point-of-care testing (POCT) applications, we used a smartphone-based portable Raman spectrometer to quantitatively analyze the SERS-LFA strips. The Raman signal could still be accurately detected when the concentration of SAA and CRP was 10 ng/mL, which is lower than the LOD required in clinical practice for most diseases. Therefore, taking into account its simple operation and short analysis time, by using a portable Raman spectrometer which can be equipped with a 5G cloud-based healthcare management system, the current strategy based on SERS-LFA provides the potential for the quick and on-site diagnosis of infectious diseases such as sepsis, which is of great significance for medical guidance on the treatment of widely spread infection-related diseases in remote areas that lack well-developed medical resources.
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