4.7 Article

Exosome-transmitted miRNA-335-5p promotes colorectal cancer invasion and metastasis by facilitating EMT via targeting RASA1

Journal

MOLECULAR THERAPY-NUCLEIC ACIDS
Volume 24, Issue -, Pages 164-174

Publisher

CELL PRESS
DOI: 10.1016/j.omtn.2021.02.022

Keywords

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Funding

  1. Natural Science Foundation of Zhejiang Province [LY19H160024]
  2. National Natural Science Foundation of China [81672385]
  3. Wenzhou Science and Technological Project [Y20160159]

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Exosomal miR-335-5p derived from metastatic CRC cells promotes invasion and metastasis of CRC cells by targeting RASA1 to facilitate EMT.
Exosomal microRNA (miRNA) secretion has been characterized as a vital factor in intercellular communication among cancer cells. However, little is known about cancer-secreted miRNAs specifically involved in metastasis of colorectal cancer (CRC). Here, we found that exosomes derived from metastatic CRC cell line SW620 promoted migration, invasion, and epithelial-mesenchymal transition (EMT) of CRC cells. The profiling of exosome miRNAs revealed that microRNA (miR)-335-5p was highly expressed in exosomes from metastatic SW620 cells compared to those derived from primary SW480 cells. miR-335-5p was transmitted from metastatic SW620 cells to CRC cells via exosomes and promoted migration, invasion, and EMT of CRC cells. Moreover, exosome-transmitted miRNA-335-5p promotes CRC cell invasion and metastasis by facilitating EMT via targeting RAS p21 protein activator 1 (RASA1). Overexpression of RASA1 abolished the promotive effects of exosomal miR-335-5p on CRC cell migration, invasion, and EMT. Collectively, our data revealed that exosomal miR-335-5p derived from metastatic CRC cells promotes CRC cell invasion and metastasis by facilitating EMT via targeting RASA1, which may serve as a potential therapeutic target for CRC metastasis.

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