Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 175, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/62619
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Funding
- National Key Research and Development Program [2018YFC1003100]
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NICS technology may offer a solution to the problems in the prevailing PGT-A method, but its efficacy has been limited. A new study reports the full protocol of NICS, including culture medium sampling, whole genome amplification, and data analysis methods. Cryopreservation times in different embryo laboratories influence the choice of embryo culture medium collection methods.
In clinical in vitro fertilization (IVF), the prevailing method for PGT-A requires biopsy of a few cells from the trophectoderm (TE). This is the lineage that forms the placenta. This method, however, requires specialized skills, is invasive, and suffers from false positives and negatives because the chromosome numbers in the TE and the inner cell mass (ICM), which develops into the fetus, are not always the same. NICS, a technology requiring sequencing of DNA that released into the culture medium from both TE and ICM, may offer a way out to these problems but has previously been shown to have limited efficacy. The present study reports the full protocol of NICS, which includes culture medium sampling methods, whole genome amplification (WGA) and library preparation, and NGS data analysis by analysis software. Considering the different cryopreservation times in different embryo laboratories, embryologists have two methods for collecting embryo culture medium that can be selected according to the actual conditions of the IVF laboratory.
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