Journal
JOURNAL OF CO2 UTILIZATION
Volume 48, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jcou.2021.101518
Keywords
Edible herbs; Puerariae lobatae; Pinus massoniana; Citrus reticulate; Supercritical fluid extraction; Antioxidant activity
Funding
- Natural Science Foundation of China [32071509, 31670631]
- Department of Science and Technology of Ningbo [2019C10094, 2019C10008, 202002N3083, 202002N3028]
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In this study, edible herbs including Puerariae lobatae root, Pinus massoniana needle, and Citrus reticulata peel were extracted using supercritical CO2 fluid instead of ethanol solution. Optimal extraction conditions were determined through experiments and data analysis, assessing antioxidant activity and quality of the natural products. The results showed that certain components exhibited high antioxidant activity, providing important guidance for extraction and evaluation of herbal natural products.
The edible herbs Puerariae lobatae root, Pinus massoniana needle, Citrus reticulata peel and their mixture SGLUAP were extracted by supercritical CO2 fluid replacing usual ethanol solution. Optimal conditions of the extraction were determined by conducting series of experiments with Box-Benhnken Design of three-variables and three-levels, data fitting and modeling, and analyzing the visual 3D response surface plots. Scavenging free radical rate (DPPH%) and inhibiting xanthine oxidase rate (XO%) of 14 major ingredients in herbs and total phenolic compounds in SGLUAP were assessed. Among them, gallic acid gave DPPH (%) of 94.5 % to be higher than the reference Vitamin C, and daidzein possessed XO (%) of 97.8 % to be much higher than the reference allopurinol. This work has demonstrated a reliable and efficient methodology for extraction optimization, antioxidant activity evaluation and quality assessment of natural products from herbs or plants.
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