Analyses of Insecticide Resistance Genes in Aedes aegypti and Aedes albopictus Mosquito Populations from Cameroon

The emergence of insecticide resistance in Aedes mosquitoes could pose major challenges for arboviral-borne disease control. In this paper, insecticide susceptibility level and resistance mechanisms were assessed in Aedes aegypti (Linnaeus, 1762) and Aedes albopictus (Skuse, 1894) from urban settings of Cameroon. The F1 progeny of Aedes aegypti and Aedes albopictus collected in Douala, Yaounde and Dschang from August to December 2020 was tested using WHO tube assays with four insecticides: deltamethrin 0.05%, permethrin 0.75%, DDT 4% and bendiocarb 0.1%. TaqMan, qPCR and RT-qPCR assays were used to detect kdr mutations and the expression profiles of eight detoxification genes. Aedes aegypti mosquitoes from Douala were found to be resistant to DDT, permethrin and deltamethrin. Three kdr mutations, F1534C, V1016G and V1016I were detected in Aedes aegypti populations from Douala and Dschang. The kdr allele F1534C was predominant (90%) in Aedes aegypti and was detected for the first time in Aedes albopictus (2.08%). P450s genes, Cyp9J28 (2.23-7.03 folds), Cyp9M6 (1.49-2.59 folds), Cyp9J32 (1.29-3.75 folds) and GSTD4 (1.34-55.3 folds) were found overexpressed in the Douala and Yaounde Aedes aegypti populations. The emergence of insecticide resistance in Aedes aegypti and Aedes albopictus calls for alternative strategies towards the control and prevention of arboviral vector-borne diseases in Cameroon.

Automated summary

The study investigated insecticide susceptibility and resistance mechanisms in Aedes mosquitoes from urban Cameroon, revealing resistance in Aedes aegypti in Douala and detection of kdr mutations. Overexpression of detoxification genes was observed in Aedes aegypti populations from Douala and Yaounde. The emergence of insecticide resistance in Aedes mosquitoes in Cameroon highlights the need for alternative strategies in arboviral vector-borne disease control.