Journal
FRONTIERS IN PHYSIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2021.674023
Keywords
Holotrichia parallela; antennal transcriptome; odorant-degrading enzyme; carboxylesterase; antenna-biased expression profile
Categories
Funding
- Jilin University [JLUXKJC2020107]
- National Key Research and Development Program of China [2018YFD0201000, 2017YFD0200600]
- Professorial and Doctoral Scientific Research Foundation of Huizhou University [2020JB069]
Ask authors/readers for more resources
In this study, 20 candidate CXEs were identified in Holotrichia parallela, with some showing antenna-biased expression. The differential expression of CXEs in male and female antennae implies potential roles in odorant degradation, providing insights into the olfactory mechanism of H. parallela.
Insects rely on their olfactory systems in antennae to recognize sex pheromones and plant volatiles in surrounding environments. Some carboxylesterases (CXEs) are odorant-degrading enzymes (ODEs), degrading odorant signals to protect the olfactory neurons against continuous excitation. However, there is no report about CXEs in Holotrichia parallela, one of the most major agricultural underground pests in China. In the present study, 20 candidate CXEs were identified based on transcriptome analysis of female and male antennae. Sequence alignments and phylogenetic analysis were performed to investigate the characterization of these candidate CXEs. The expression profiles of CXEs were compared by RT-qPCR analysis between olfactory and non-olfactory tissues of both genders. HparCXE4, 11, 16, 17, 18, 19, and 20 were antenna-biased expressed genes, suggesting their possible roles as ODEs. HparCXE6, 10, 11, 13, and 16 showed significantly higher expression profiles in male antennae, whereas HparCXE18 was expressed more in female antennae. This study highlighted candidate CXE genes linked to odorant degradation in antennae, and provided a useful resource for further work on the H. parallela olfactory mechanism and selection of target genes for integrative control of H. parallela.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available