Journal
SUSTAINABILITY
Volume 13, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/su13116006
Keywords
bioproducts; Penicillium oxalicum; biodegradation; cellulases
Funding
- National Key R and D Program of China [2017YFD0600205]
- Fundamental Research Funds for the Central Universities [2572019CZ01, 2572019EA01, 2572019AA17]
- 111 Project [B20088]
- Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team)
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Endophytic fungi can degrade plant cellulose, and endophytic Penicillium has high cellulase activity. Endophytic fungi in special natural environments offer new directions for future industrial enzyme research.
Endophytic fungi inside a plant can degrade a portion of plant lignin and cellulose. Endophytic Penicillium is one of the industrial microorganisms with the advantage of producing enzymes with a complete enzyme system that can be secreted into the extracellular space. The natural evolution of ancient tree species from special natural geographic environments to screen out cellulase-producing strains with excellent characteristics provides a promising direction for future industrial enzymes. The present study successfully isolated and screened a novel fungal endophyte, Penicillium oxalicum R4, with higher cellulase activity from Taxus cuspidata. Under the optimized culture conditions obtained by a Box-Behnken design (BBD) and an artificial neural network-genetic algorithm (ANN-GA), yields of Filter Paperase (FPase), Carboxymethyl Cellulase (CMCase) and beta-glucosidase (beta GLase) produced by P. oxalicum R4 were 1.45, 5.27 and 6.35 U/mL, which were approximately 1.60-fold, 1.59-fold and 2.16-fold higher than those of the non-optimized culture, respectively. The discovery of cellulase-producing strains of endophytic fungi located in special natural geographic environments, such as Taxus cuspidata, which is known as a living plant fossil, provides new research directions for future industrial enzymes.
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