Fabrication of Microparticles with Front-Back Asymmetric Shapes Using Anisotropic Gelation

Droplet-based microfluidics is a powerful tool for producing monodispersed micrometersized droplets with controlled sizes and shapes; thus, it has been widely applied in diverse fields from fundamental science to industries. Toward a simpler method for fabricating microparticles with front-back asymmetry in their shapes, we studied anisotropic gelation of alginate droplets, which occurs inside a flow-focusing microfluidic device. In the proposed method, sodium alginate (NaAlg) aqueous phase fused with a calcium chloride (CaCl2) emulsion dispersed in the organic phase just before the aqueous phase breaks up into the droplets. The fused droplet with a front-back asymmetric shape was generated, and the asymmetric shape was kept after geometrical confinement by a narrow microchannel was removed. The shape of the fused droplet depended on the size of prefused NaAlg aqueous phase and a CaCl2 emulsion, and the front-back asymmetry appeared in the case of the smaller emulsion size. The analysis of the velocity field inside and around the droplet revealed that the stagnation point at the tip of the aqueous phase also played an important role. The proposed mechanism will be potentially applicable as a novel fabrication technique of microparticles with asymmetric shapes.

Automated summary

Droplet-based microfluidics has been widely used for producing monodispersed micrometer-sized droplets with controlled sizes and shapes. A simplified method for fabricating microparticles with front-back asymmetry in their shapes was studied by investigating anisotropic gelation of alginate droplets, leading to potential applications in novel fabrication techniques of microparticles with asymmetric shapes.