Journal
LIGHT-SCIENCE & APPLICATIONS
Volume 10, Issue 1, Pages -Publisher
SPRINGERNATURE
DOI: 10.1038/s41377-021-00587-6
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Funding
- National Natural Science Foundation of China [62088102, 62071272, 61927802]
- National Key Research and Development Program of China [2020AAA0130000]
- Postdoctoral Science Foundation of China [2019M660644]
- Tsinghua University Initiative Scientific Research Program
- Beijing Laboratory of Brain and Cognitive Intelligence, Beijing Municipal Education Commission
- National Postdoctoral Program for Innovative Talent and Shuimu Tsinghua Scholar Program
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The DiLFM technique, utilizing an over-complete dictionary, effectively suppresses reconstruction artifacts and improves noise robustness. The study demonstrates the application of DiLFM in scattering samples such as Drosophila embryos and brains.
Dictionary light-field microscopy (DiLFM) substantially eliminates various kinds of reconstruction artifacts and improves the noise robustness. Using DiLFM yields accurate observation of zebrafish blood flow at 100 Hz in low-light conditions. Light field microscopy (LFM) has been widely used for recording 3D biological dynamics at camera frame rate. However, LFM suffers from artifact contaminations due to the illness of the reconstruction problem via naive Richardson-Lucy (RL) deconvolution. Moreover, the performance of LFM significantly dropped in low-light conditions due to the absence of sample priors. In this paper, we thoroughly analyze different kinds of artifacts and present a new LFM technique termed dictionary LFM (DiLFM) that substantially suppresses various kinds of reconstruction artifacts and improves the noise robustness with an over-complete dictionary. We demonstrate artifact-suppressed reconstructions in scattering samples such as Drosophila embryos and brains. Furthermore, we show our DiLFM can achieve robust blood cell counting in noisy conditions by imaging blood cell dynamic at 100 Hz and unveil more neurons in whole-brain calcium recording of zebrafish with low illumination power in vivo.
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