Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
Volume 16, Issue -, Pages 38-44Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpddr.2021.04.004
Keywords
Trichomonas vaginalis; Tritrichomonas foetus; Metronidazole resistance; Oxygen scavenging; Flavin reductase
Categories
Funding
- University of Veterinary Medicine Vienna
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Trichomonads are a group of parasitic protists infecting various hosts, with Trichomonas vaginalis and Tritrichomonas foetus being medically and veterinary important. 5-nitroimidazoles like metronidazole are effective against trichomonads due to their microaerophilic/anaerobic nature. Drug resistance, particularly in T. vaginalis, has been linked to defective oxygen scavenging mechanisms, specifically involving flavin reductase and NADH oxidase. However, the involvement of these enzymes in metronidazole resistance remains unclear in T. foetus.
Trichomonads are an order of parasitic protists which infect a wide range of hosts. The human parasite Trichomonas vaginalis and the bovine parasite Tritrichomonas foetus which also infects cats and swine are of considerable medical and veterinary importance, respectively. Since trichomonads are microaerophiles/anaerobes they are susceptible to 5-nitroimidazoles such as metronidazole. 5-nitroimidazoles are exclusively toxic to microaerophilic/anaerobic organisms because reduction, i.e. activation, of the drug can only occur in a highly reductive environment. 5-nitroimidazoles have remained a reliable treatment option throughout the last decades but drug resistance can be a problem. Clinical resistance to 5-nitroimidazoles has been studied in more detail in T. vaginalis and has been ascribed to defective oxygen scavenging mechanisms which lead to higher intracellular oxygen concentrations and, consequently, to less drug being reduced. Two enzymes, flavin reductase (FR) and NADH oxidase have been suggested to be the major oxygen scavenging enzymes in T. vaginalis. The loss, or at least an impairment of FR which reduces oxygen to hydrogen peroxide, has been proposed as the central mechanism that enables the emergence of 5-nitroimidazole resistance. In this study we explored if T. foetus also encodes a homolog of FR and if it is, likewise, involved in resistance. T. foetus was indeed found to express a FR but it was only weakly active as compared to the T. vaginalis homolog. Further, activity of FR in T. foetus was unchanged in metronidazole-resistant cell lines, ruling out that it has a role in metronidazole resistance. Finally, we measured oxygen scavenging rates in metronidazole-sensitive and -resistant cell lines and found that NADH oxidase and FR are not the major oxygen scavenging enzymes in trichomonads and that oxygen scavenging is possibly a consequence, rather than a cause of metronidazole resistance.
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