Journal
GENOME
Volume 59, Issue 3, Pages 173-183Publisher
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/gen-2015-0115
Keywords
disease resistance; Fusarium crown and root rot; Fusarium oxysporum f. sp radicis-lycopersici; molecular marker; Solanum lycopersicum
Funding
- Golden Seed Project (Center for Horticultural Seed Development)
- Ministry of Agriculture, Food and Rural Affairs (MAFRA)
- Ministry of Oceans and Fisheries (MOF)
- Rural Development Administration (RDA)
- Korea Forest Service (KFS)
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Fusarium crown and root rot is a severe fungal disease of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL). In this study, the genomic location of the FORL-resistance locus was determined using a set of molecular markers on chromosome 9 and an F-2 population derived from FORL-resistant inbred 'AV107-4' (Solanum lycopersicum) x susceptible 'L3708' (Solanum pimpinellifolium). Bioassay performed using Korean FORL strain KACC 40031 showed single dominant inheritance of FORL resistance in the F2 population. In all, 13 polymerase chain reaction-based markers encompassing approximately 3.6-72.0 Mb of chromosome 9 were developed based on the Tomato-EXPEN 2000 map and SolCAP Tomato single nucleotide polymorphism array analysis. These markers were genotyped on 345 F2 plants, and the FORL-resistance locus was found to be present on a pericentromeric region of suppressed chromosomal recombination in chromosome 9. The location of the FORL-resistance locus was further confirmed by testing these markers against diverse commercial tomato and stock cultivars resistant to FORL. A restriction fragment length polymorphism marker, PNU-D4, located at approximately 6.1 Mb of chromosome 9 showed the highest match with the resistance locus and was used for conducting high-resolution melting analysis for marker-assisted selection of FORL resistance.
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