4.7 Article

OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling

Journal

FRONTIERS IN PLANT SCIENCE
Volume 12, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.699303

Keywords

rice; OsNAC2; ethylene; abscisic acid; seed germination; seedling growth

Categories

Funding

  1. Ministry of Agriculture of the People's Republic of China [2016ZX08009-001-008]
  2. National Key R&D Program of China [2018YFD1000400]
  3. Science and Technology Commission of Shanghai Municipality [18DZ2260500]
  4. Shanghai Engineering Research Center of Plant Germplasm Resources [17DZ2252700]
  5. National Natural Science Foundation of China [31801321]
  6. Natural Science Foundation of Shanghai [20ZR1441000]

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This study demonstrated that the rice NAC transcription factor OsNAC2 increased ethylene sensitivity in rice roots during the seedling period, delaying seedling establishment by enhancing ethylene synthesis. OsNAC2 also delayed seed germination and coleoptile growth through the ABA pathway, and regulated downstream targets in a time-dependent manner by binding to the promoter of OsKO2 in the seedling period.
The germination of seeds and establishment of seedling are the preconditions of plant growth and are antagonistically regulated by multiple phytohormones, e.g., ethylene, abscisic acid (ABA), and gibberellic acid (GA). However, the interactions between these phytohormones and their upstream transcriptional regulation during the seed and seedling growth in rice remain poorly understood. Here, we demonstrated a rice NAC (NAM-ATAF-CUC) transcription factor, OsNAC2, the overexpression of which increases the ethylene sensitivity in rice roots during the seedling period. Further study proved that OsNAC2 directly activates the expressions of OsACO and OsACO3, enhancing ethylene synthesis, and then retards seedling establishment. Moreover, OsNAC2 delays the germination of seeds and coleoptile growth through the ABA pathway instead of the ethylene and GA pathway, by targeting the promoters of OsNCED3, OsZEP1, and OsABA8ox1. We also found that OsNAC2 regulates downstream targets in a time-dependent manner by binding to the promoter of OsKO2 in the seedling period but not in the germination stage. Our finding enriched the regulatory network of ethylene, ABA, and GA in the germination of rice seeds and seedling growth, and uncovered new insights into the difference of transcription factors in targeting their downstream components.

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