4.7 Article

Hydrogen Sulfide Alleviates Alkaline Salt Stress by Regulating the Expression of MicroRNAs in Malus hupehensis Rehd. Roots

Journal

FRONTIERS IN PLANT SCIENCE
Volume 12, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.663519

Keywords

Malus hupehensis Rehd; microRNAs; hydrogen sulfide; alkaline salt stress; BGISEQ-500

Categories

Funding

  1. National Natural Science Foundation of China [31772251]
  2. National Key R&D Program of China [2019YFD1000103]
  3. Shandong Province Natural Science Foundation [ZR2018ZC08N3]
  4. Major Project of Science and Technology of Shandong Province [2018CXGC0207]

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The study identified 115 known miRNAs and 15 predicted novel miRNAs in Malus hupehensis roots, as well as analyzed 175 miRNA target genes. By validating the expression levels of miRNAs and target genes, it was found that H2S pretreatment specifically regulated certain miRNAs and improved root architecture. Overall, the findings suggest that a miRNA-mediated regulatory network is involved in the H2S mitigation of alkaline salt stress in Malus hupehensis roots.
Malus hupehensis Rehd. var. pingyiensis Jiang (Pingyi Tiancha, PYTC) is an excellent apple rootstock and ornamental tree, but its tolerance to salt stress is weak. Our previous study showed that hydrogen sulfide (H2S) could alleviate damage in M. hupehensis roots under alkaline salt stress. However, the molecular mechanism of H2S mitigation alkaline salt remains to be elucidated. MicroRNAs (miRNAs) play important regulatory roles in plant response to salt stress. Whether miRNAs are involved in the mitigation of alkaline salt stress mediated by H2S remains unclear. In the present study, through the expression analysis of miRNAs and target gene response to H2S and alkaline salt stress in M. hupehensis roots, 115 known miRNAs (belonging to 37 miRNA families) and 15 predicted novel miRNAs were identified. In addition, we identified and analyzed 175 miRNA target genes. We certified the expression levels of 15 miRNAs and nine corresponding target genes by real-time quantitative PCR (qRT-PCR). Interestingly, H2S pretreatment could specifically induce the downregulation of mhp-miR408a expression, and upregulated mhp-miR477a and mhp-miR827. Moreover, root architecture was improved by regulating the expression of mhp-miR159c and mhp-miR169 and their target genes. These results suggest that the miRNA-mediated regulatory network participates in the process of H2S-mitigated alkaline salt stress in M. hupehensis roots. This study provides a further understanding of miRNA regulation in the H2S mitigation of alkaline salt stress in M. hupehensis roots.

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