Journal
FRONTIERS IN MICROBIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.707711
Keywords
Pseudomonas aeruginosa; two-component system; FleS/FleR; biofilm; swimming motility
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Funding
- Key Projects of Guangzhou Science and Technology Plan [201804020066]
- Natural Research Foundation of China [31330002]
- Key Realm R&D Program of Guangdong Province [2018B020205003, 2020B0202090001]
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Pseudomonas aeruginosa, a major cause of nosocomial infection, exhibits great adaptive ability under diverse environmental conditions, regulated by multiple signaling systems such as the two-component system (TCS) including FleS/FleR. This study identified key residues and regulatory roles of FleS/FleR in virulence-related traits and revealed their involvement in biofilm formation and flagellum biosynthesis. The findings provide insights into the mechanisms of regulation by FleS/FleR in P. aeruginosa.
Pseudomonas aeruginosa, a major cause of nosocomial infection, can survive under diverse environmental conditions. Its great adaptive ability is dependent on its multiple signaling systems such as the two-component system (TCS). A TCS FleS/FleR has been previously identified to positively regulate a variety of virulence-related traits in P. aeruginosa PAO1 including motility and biofilm formation which are involved in the acute and chronic infections, respectively. However, the molecular mechanisms underlying these regulations are still unclear. In this study, we first analyzed the regulatory roles of each domains in FleS/FleR and characterized key residues in the FleS-HisKA, FleR-REC and FleR-AAA domains that are essential for the signaling. Next, we revealed that FleS/FleR regulates biofilm formation in a c-di-GMP and FleQ dependent manner. Lastly, we demonstrated that FleR can regulate flagellum biosynthesis independently without FleS, which explains the discrepant regulation of swimming motility by FleS and FleR.
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