Journal
FRONTIERS IN MICROBIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.682829
Keywords
chitosanase; Bacillus atrophaeus; chitooligosaccharide; antifungal activity; hydrolysis rate
Categories
Funding
- National Key Research and Development Program of China [2016YFD0200405-5]
- Science and Technology Planning Project of Guangdong [2019B030301007]
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The study characterized a potential GH46 family chitosanase, Csn-SH, from Bacillus atrophaeus BSS, which exhibited efficient hydrolysis activity towards chitosan and good antifungal properties. The findings suggest that Csn-SH could be utilized for the efficient production of bioactive chitooligosaccharides for safe and sustainable agricultural practices.
Chitosanase plays a vital role in bioactive chitooligosaccharide preparation. Here, we characterized and prepared a potential GH46 family chitosanase from Bacillus atrophaeus BSS. The purified recombinant enzyme Csn-SH showed a molecular weight of 27.0 kDa. Csn-SH displayed maximal activity toward chitosan at pH 5.0 and 45 degrees C. Thin-layer chromatography and electrospray ionization-mass spectrometry indicated that Csn-SH mainly hydrolyzed chitosan into (GlcN)(2), (GlcN)(3), and (GlcN)(4) with an endo-type cleavage pattern. Molecular docking analysis demonstrated that Csn-SH cleaved the glycoside bonds between subsites -2 and + 1 of (GlcN)(6). Importantly, the chitosan hydrolysis rate of Csn-SH reached 80.57% within 40 min, which could reduce time and water consumption. The hydrolysates prepared with Csn-SH exhibited a good antifungal activity against Magnaporthe oryzae and Colletotrichum higginsianum. The above results suggested that Csn-SH could be used to produce active chitooligosaccharides efficiently that are biocontrol agents applicable for safe and sustainable agricultural production.
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