Journal
GENETICS AND MOLECULAR RESEARCH
Volume 15, Issue 3, Pages -Publisher
FUNPEC-EDITORA
DOI: 10.4238/gmr.15037868
Keywords
Endoplasmic reticulum (ER) stress; Tunicamycin (TM); Goose primary hepatocytes; Unfolded protein response (UPR); PI3k-Akt-mTOR signaling pathways
Funding
- National Natural Science Funds of China [31101712]
- Research Fund for the Doctoral Program of Higher Education of China [20115103120006]
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PI3K-Akt-mTOR signaling pathway is associated with endoplasmic reticulum (ER) stress. However, it is not clear how this signaling pathway affects the ER stress. The present study aimed to determine whether the PI3K-Akt-mTOR signaling pathway regulates tunicamycin (TM)-induced increases in mRNA levels of genes involved in the ER stress, to help elucidate the mechanism by which this pathway affects the ER stress in primary goose hepatocytes. Primary hepatocytes were isolated from geese and cultured in vitro. After 12 h in a serum-free medium, the hepatocytes were incubated for 24 h in a medium with either no addition (control) or with supplementation of TM or TM together with PI3K-Akt-mTOR signaling pathway inhibitors (LY294002, rapamycin, NVP-BEZ235). Thereafter, the expression levels of genes involved in the ER stress (BIP, EIF2a, ATF6, and XBP1) were assessed. The results indicated that the mRNA level of BIP was up-regulated in 0.2, 2, and 20 mu M TM treatment group (P < 0.05), whereas the mRNA levels of EIF2a, ATF6, and XBP1 were up-regulated in the 2 mu M TM treatment group (P < 0.05). However, the TM mediated induction of mRNA levels of genes involved in the ER stress (BIP, EIF2a, ATF6, and XBP1) was down-regulated after the treatment with PI3K-Akt-mTOR pathway inhibitors (LY294002, NVP-BEZ235, and rapamycin). Therefore, our results strongly suggest that the PI3K-Aktm-TOR signaling pathway might be involved in the down-regulation of the TM-induced ER stress in primary goose hepatocytes.
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