LSTMCNNsucc: A Bidirectional LSTM and CNN-Based Deep Learning Method for Predicting Lysine Succinylation Sites

Lysine succinylation is a typical protein post-translational modification and plays a crucial role of regulation in the cellular process. Identifying succinylation sites is fundamental to explore its functions. Although many computational methods were developed to deal with this challenge, few considered semantic relationship between residues. We combined long short-term memory (LSTM) and convolutional neural network (CNN) into a deep learning method for predicting succinylation site. The proposed method obtained a Matthews correlation coefficient of 0.2508 on the independent test, outperforming state of the art methods. We also performed the enrichment analysis of succinylation proteins. The results showed that functions of succinylation were conserved across species but differed to a certain extent with species. On basis of the proposed method, we developed a user-friendly web server for predicting succinylation sites.

Automated summary

The study utilized a deep learning approach combining LSTM and CNN to predict succinylation sites, achieving excellent performance; the functions of succinylation proteins were found to be conserved across species but had some differences between species; a user-friendly web server for predicting succinylation sites was developed based on the proposed method.