4.6 Article

Streamlining Design, Engineering, and Applications of Enzymes for Sustainable Biocatalysis

Related references

Note: Only part of the references are listed.
Review Chemistry, Multidisciplinary

Biocatalysis: Enzymatic Synthesis for Industrial Applications

Shuke Wu et al.

Summary: Biocatalysis is widely used as an alternative to chemical catalysis in various fields, with enzymes playing a key role in organic synthesis, particularly in the production of chiral compounds for pharmaceuticals and flavors. Biocatalysts are also utilized on a large scale for the production of specialty and bulk chemicals. This review focuses on scalable chemical production using enzymes, discussing opportunities, limitations, and emerging enzyme classes through illustrative examples.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Review Biotechnology & Applied Microbiology

Enzyme co-immobilization: Always the biocatalyst designers' choice ... or not? ...

Sara Arana-Pena et al.

Summary: Enzyme co-immobilization offers kinetic advantages but also drawbacks, such as the need to discard the entire biocatalyst if one enzyme becomes inactivated. The co-immobilization of cofactors and enzymes is of particular interest for the construction of artificial cells and complex one-pot transformations.

BIOTECHNOLOGY ADVANCES (2021)

Article Chemistry, Applied

Development of a Nitrene-Type Rearrangement for the Commercial Route of the JAK1 Inhibitor Abrocitinib

Christina G. Connor et al.

Summary: The development of a commercial route towards the JAK1 inhibitor abrocitinib involves a late-stage Lossen rearrangement to obtain the desired compound, which is then sulfonylated using a novel water-tolerable triazole sulfonylating reagent to provide the active pharmaceutical ingredient.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2021)

Review Chemistry, Multidisciplinary

Navigating the Unnatural Reaction Space: Directed Evolution of Heme Proteins for Selective Carbene and Nitrene Transfer

Yang Yang et al.

Summary: Researchers are making efforts to expand the catalytic repertoire of enzymes to include powerful reactions previously only known in small-molecule catalysis. By leveraging the catalytic promiscuity of P450 enzymes, a range of carbene and nitrene transferases with excellent activity towards new-to-nature reactions have been evolved.

ACCOUNTS OF CHEMICAL RESEARCH (2021)

Article Chemistry, Physical

Integrated Electro-Biocatalysis for Amine Alkylation with Alcohols

Itziar Penafiel et al.

Summary: The integration of electro and bio-catalysis offers new ways of making molecules under very mild, environmentally benign conditions. The combined process offers a new approach to amine alkylation with native alcohols, a key bond formation in the chemical economy that is currently achieved via precious metal-catalyzed hydrogen-borrowing technologies.

CHEMCATCHEM (2021)

Review Biochemistry & Molecular Biology

Flow Biocatalysis: A Challenging Alternative for the Synthesis of APIs and Natural Compounds

Micol Santi et al.

Summary: Biocatalysts have become a popular choice in the past few years, especially when applied in continuous flow systems, which are crucial for overcoming the limitations of traditional enzymatic catalysis and enabling more sustainable synthetic pathways.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biochemical Research Methods

Biocatalysis - Key enabling tools from biocatalytic one-step and multi-step reactions to biocatalytic total synthesis

Roland Wohlgemuth

Summary: Biocatalysis has had a significant impact in improving quality of life, with key tools providing opportunities for further development and applications in various sectors of the global bioeconomy. Systems biocatalysis and integration with sustainable chemistry in vitro aim at high molecular economy conversions, while tools such as bioinformatic tools and enzyme engineering are important for rapid prototyping of biocatalytic reactions and designing multi-step reactions.

NEW BIOTECHNOLOGY (2021)

Article Chemistry, Organic

Expanding the synthetic scope of biocatalysis by enzyme discovery and protein engineering

James R. Marshall et al.

Summary: The full potential of enzyme catalysis in industrial chemistry has yet to be unlocked, but accessing new chemistries and expanding existing reactions are necessary for making biocatalysis a pivotal technology in chemical manufacturing. Recently, the biocatalytic toolbox for synthetic chemistry has been expanded by extending the scope of industrially relevant reactions and adding new reactions through enzyme discovery or protein engineering.

TETRAHEDRON (2021)

Review Engineering, Chemical

Multi-Enzyme Systems in Flow Chemistry

Pedro Fernandes et al.

Summary: In recent years, there has been a growing interest in using biocatalysts in flow reactors, combining the high selectivity and mild operation conditions of biocatalysis with the advantages of flow chemistry. This approach allows for efficient cascade reactions, mimics natural metabolic pathways, and provides opportunities for novel production pathways.

PROCESSES (2021)

Article Chemistry, Physical

RetroBioCat as a computer-aided synthesis planning tool for biocatalytic reactions and cascades

William Finnigan et al.

Summary: With the expanding enzyme toolbox for biocatalysis, there is potential for constructing powerful enzymatic cascades for efficient and selective synthesis of target molecules. RetroBioCat is an intuitive tool for computer-aided design of biocatalytic cascades, utilizing expertly encoded reaction rules and literature precedent to identify promising biocatalytic pathways.

NATURE CATALYSIS (2021)

Review Chemistry, Multidisciplinary

Fatty Acids and their Derivatives as Renewable Platform Molecules for the Chemical Industry

Ursula Biermann et al.

Summary: Oils and fats of vegetable and animal origin are important renewable feedstock for the chemical industry, with their industrial use increasing significantly in the past decade. Remarkable achievements in oleochemistry include reduction of fatty esters, selective oxidation of double bonds, synthesis of alkyl-branched compounds, and applications in the synthesis of polymeric materials.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Article Chemistry, Physical

Efficient Acylation of Sugars and Oligosaccharides in Aqueous Environment Using Engineered Acyltransferases

Simon P. Godehard et al.

Summary: One major challenge in enzymatic synthesis of sugar esters is the low solubility of sugars in toxic organic solvents. This study demonstrated efficient acetylation of sugars in water using engineered acyltransferases, achieving high conversion rates for glucose, maltose, and maltotriose. The discovery of EstA as a promiscuous acyltransferase that preferentially acetylates sugars expands the potential applications of such enzymes in sugar modifications.

ACS CATALYSIS (2021)

Article Chemistry, Multidisciplinary

Engineering the Performance of Artificial Inclusion Bodies Built of Catalytic β-Galactosidase

Julieta M. Sanchez et al.

Summary: A self-immobilized enzymatic platform based on Escherichia coli beta-galactosidase was designed, showing enhanced thermal stability and high reusability. The use of divalent cations as molecular linkers in assembling stable protein microparticles offered efficient catalytic performance. The platform presents transversal applicability and great potential in biotechnology and biomedicine.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2021)

Article Chemistry, Physical

Magnetic Cross-Linked Enzyme Aggregates of a Transpeptidase-Specialized Variant (N450D) of Bacillus licheniformis gamma-Glutamyl Transpeptidase: An Efficient and Stable Biocatalyst for l-Theanine Synthesis

Meng-Chun Chi et al.

Summary: In this study, magnetic cross-linked enzyme aggregates (mCLEAs) of a transpeptidase-specialized variant (N450D) of Bacillus licheniformis GGT were successfully prepared using optimized process parameters. The immobilized enzyme exhibited enhanced thermal and storage stability compared to the free enzyme, and could be reused for multiple cycles without significant reduction in product yield, showing potential for biocatalysis in the production of physiologically important gamma-glutamyl compounds.

CATALYSTS (2021)

Article Chemistry, Physical

Engineering actively magnetic crosslinked inclusion bodies of Candida antarctica lipase B: An efficient and stable biocatalyst for enzyme-catalyzed reactions

Yu Han et al.

Summary: This study demonstrates that recombinant Candida antarctica lipase B expressed in the form of inclusion bodies remains active and can be crosslinked with oxidized dextran to form magnetic aggregates with high activity recovery. These aggregates show excellent thermal stability, storage stability, and pH tolerance, while also exhibiting the ability to be recycled effectively and retain high activity levels. Additionally, they can be used in carbon-carbon bond-forming reactions to produce multiple products simultaneously, showcasing their potential as a versatile biocatalyst.

MOLECULAR CATALYSIS (2021)

Review Biochemical Research Methods

Enzyme evolution for industrial biocatalytic cascades

Jovana Nazor et al.

Summary: Multi-step, biocatalytic cascades show great potential in leading the further adoption of enzymes in the chemical industry by enabling difficult chemistry under mild reaction conditions with minimal waste generation. Recent advances in biocatalysis have led to increasingly complex cascades, optimized through enzyme engineering and evolution to improve activity, selectivity, and stability. Industrially relevant enzyme cascades have been successfully developed with the aim of sustainable and economically attractive biocatalytic synthesis of complex chemicals.

CURRENT OPINION IN BIOTECHNOLOGY (2021)

Review Chemistry, Multidisciplinary

Concepts for flow chemistry with whole-cell biocatalysts

Niklas Adebar et al.

Summary: By combining continuous flow processing and biocatalysis, efficient, stable, and cost-effective processes can be achieved. Recent years have seen significant progress in the rational design of recombinant whole-cell catalysts. The combination of these powerful catalysts with continuous reactor concepts is emerging, with applications in packed bed reactors, microchannels, and biofilm reactors.

REACTION CHEMISTRY & ENGINEERING (2021)

Review Chemistry, Multidisciplinary

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

Ge Qu et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Biochemistry & Molecular Biology

Combined cross-linked enzyme aggregates of glycerol dehydrogenase and NADH oxidase for high efficiency in situ NAD+ regeneration

Meng-Qiu Xu et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Review Engineering, Chemical

Syngas Fermentation to Alcohols: Reactor Technology and Application Perspective

I. Katharina Stoll et al.

CHEMIE INGENIEUR TECHNIK (2020)

Article Multidisciplinary Sciences

Reduction of nitroarenes by magnetically recoverable nitroreductase immobilized on Fe3O4 nanoparticles

Qikun Zhang et al.

SCIENTIFIC REPORTS (2020)

Article Chemistry, Multidisciplinary

Sequence-Based Prediction of Promiscuous Acyltransferase Activity in Hydrolases

Henrik Mueller et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Chemistry, Multidisciplinary

Aromas flow: eco-friendly, continuous, and scalable preparation of flavour esters

Martina Letizia Contente et al.

JOURNAL OF FLOW CHEMISTRY (2020)

Review Biotechnology & Applied Microbiology

Catalytically-active inclusion bodies for biotechnology-general concepts, optimization, and application

Vera D. Jaeger et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

Preparation of CLEAs and magnetic CLEAs of a recombinant L-arabinose isomerase for D-tagatose synthesis

Marylane de Sousa et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

Article Chemistry, Applied

The Effect of Dissolved Oxygen on Kinetics during Continuous Biocatalytic Oxidations

Rowan M. Lindeque et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2020)

Article Multidisciplinary Sciences

Biocatalysis and biomass conversion: enabling a circular economy

Roger A. Sheldon

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES (2020)

Article Chemistry, Physical

Embracing Nature's Catalysts: A Viewpoint on the Future of Biocatalysis

Bernhard Hauer

ACS CATALYSIS (2020)

Article Chemistry, Physical

Efficient Chemo-Enzymatic Flow Synthesis of High Value Amides and Esters

Francesca Annunziata et al.

CATALYSTS (2020)

Review Chemistry, Multidisciplinary

Pharmaceutical industry perspectives on flow chemocatalysis and biocatalysis

Laura Leemans Martin et al.

CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY (2020)

Review Chemistry, Multidisciplinary

Characterization and evaluation of immobilized enzymes for applications in flow reactors

Juan M. Bolivar et al.

CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY (2020)

Review Chemistry, Multidisciplinary

The rise of continuous flow biocatalysis - fundamentals, very recent developments and future perspectives

Piera De Santis et al.

REACTION CHEMISTRY & ENGINEERING (2020)

Article Biotechnology & Applied Microbiology

Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors-Multi-step reactions and upscaling

Eduardo J. S. Bras et al.

JOURNAL OF BIOTECHNOLOGY (2020)

Article Chemistry, Multidisciplinary

Green chemistry and the plastic pollution challenge: towards a circular economy

Roger A. Sheldon et al.

GREEN CHEMISTRY (2020)

Article Chemistry, Multidisciplinary

3D printing of PEEK reactors for flow chemistry and continuous chemical processing

Matthew J. J. Harding et al.

REACTION CHEMISTRY & ENGINEERING (2020)

Article Chemistry, Multidisciplinary

The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis

Roger A. A. Sheldon et al.

CHEMICAL SCIENCE (2020)

Article Chemistry, Physical

Enzyme-Catalyzed Synthesis of Esters in Water

Luuk Mestrom et al.

CHEMCATCHEM (2019)

Article Chemistry, Inorganic & Nuclear

Magnetic cross-linked enzyme aggregates (MCLEAs) applied to biomass conversion

Guilherme Nunes Lucena et al.

JOURNAL OF SOLID STATE CHEMISTRY (2019)

Review Chemistry, Applied

Biocatalysis: A Pharma Perspective

Joseph P. Adams et al.

ADVANCED SYNTHESIS & CATALYSIS (2019)

Review Biotechnology & Applied Microbiology

Accelerating the implementation of biocatalysis in industry

John M. Woodley

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2019)

Review Chemistry, Physical

Tailoring Multipurpose Biocatalysts via Protein Engineering Approaches: A Review

Muhammad Bilal et al.

CATALYSIS LETTERS (2019)

Review Chemistry, Multidisciplinary

Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis

Roger A. Sheldon et al.

CHEMSUSCHEM (2019)

Article Agriculture, Multidisciplinary

Efficient Enzymatic Preparation of Flavor Esters in Water

Igor Chiarelli Perdomo et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2019)

Article Chemistry, Multidisciplinary

Stereodivergent Protein Engineering of a Lipase To Access All Possible Stereoisomers of Chiral Esters with Two Stereocenters

Jian Xu et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2019)

Article Chemistry, Applied

Technical Considerations for Scale-Up of Imine-Reductase-Catalyzed Reductive Amination: A Case Study

Amin Bornadel et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2019)

Article Multidisciplinary Sciences

Machine learning-assisted directed protein evolution with combinatorial libraries

Zachary Wu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Review Biochemical Research Methods

Machine-learning-guided directed evolution for protein engineering

Kevin K. Yang et al.

NATURE METHODS (2019)

Editorial Material Chemistry, Physical

Biocatalysis and Pharmaceuticals: A Smart Tool for Sustainable Development

Andres R. Alcantara

CATALYSTS (2019)

Editorial Material Chemistry, Physical

BIOCATALYSIS Engineering imine reductases for industrial applications

Matthias Hoehne

NATURE CATALYSIS (2019)

Article Biochemistry & Molecular Biology

An Engineered Alcohol Oxidase for the Oxidation of Primary Alcohols

Rachel S. Heath et al.

CHEMBIOCHEM (2019)

Review Chemistry, Applied

Biocatalysis Using Immobilized Enzymes in Continuous Flow for the Synthesis of Fine Chemicals

Matthew P. Thompson et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2019)

Review Biochemistry & Molecular Biology

Trends on enzyme immobilization researches based on bibliometric analysis

Maria Carolina Pereira Goncalves et al.

PROCESS BIOCHEMISTRY (2019)

Article Chemistry, Organic

A generic platform for the immobilisation of engineered biocatalysts

Matthew P. Thompson et al.

TETRAHEDRON (2019)

Article Multidisciplinary Sciences

Design of an invitro biocatalytic cascade for the manufacture of islatravir

Mark A. Huffman et al.

SCIENCE (2019)

Review Chemistry, Physical

Industrial applications of immobilized enzymes-A review

Alessandra Basso et al.

MOLECULAR CATALYSIS (2019)

Article Biotechnology & Applied Microbiology

Metabolic Engineering and Synthetic Biology: Synergies, Future, and Challenges

Raul Garcia-Granados et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2019)

Review Chemistry, Inorganic & Nuclear

Recent Advances of Cellulase Immobilization onto Magnetic Nanoparticles: An Update Review

Kamyar Khoshnevisan et al.

MAGNETOCHEMISTRY (2019)

Review Chemistry, Physical

A comprehensive metabolic map for production of bio-based chemicals

Sang Yup Lee et al.

NATURE CATALYSIS (2019)

Article Biotechnology & Applied Microbiology

Bioengineering toward direct production of immobilized enzymes: A paradigm shift in biocatalyst design

Fabian B. H. Rehm et al.

BIOENGINEERED (2018)

Article Chemistry, Physical

Polyaniline-coated magnetic diatomite nanoparticles as a matrix for immobilizing enzymes

Mariana Paola Cabrera et al.

APPLIED SURFACE SCIENCE (2018)

Article Biochemistry & Molecular Biology

Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates

Huihua Sun et al.

BIOORGANIC & MEDICINAL CHEMISTRY (2018)

Review Biochemistry & Molecular Biology

Biocatalysts for the pharmaceutical industry created by structure-guided directed evolution of stereoselective enzymes

Guangyue Li et al.

BIOORGANIC & MEDICINAL CHEMISTRY (2018)

Article Biotechnology & Applied Microbiology

Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

Caterina Martin et al.

BIOTECHNOLOGY FOR BIOFUELS (2018)

Article Chemistry, Physical

Hydrogen-Borrowing Alcohol Bioamination with Coimmobilized Dehydrogenases

Wesley Bohmer et al.

CHEMCATCHEM (2018)

Article Engineering, Environmental

Machine learning meets continuous flow chemistry: Automated optimization towards the Pareto front of multiple objectives

Artur M. Schweidtmann et al.

CHEMICAL ENGINEERING JOURNAL (2018)

Review Chemistry, Multidisciplinary

Continuous flow biocatalysis

Joshua Britton et al.

CHEMICAL SOCIETY REVIEWS (2018)

Review Biochemistry & Molecular Biology

Synthesis of chiral amines using redox biocatalysis

Gideon Grogan

CURRENT OPINION IN CHEMICAL BIOLOGY (2018)

Review Biochemistry & Molecular Biology

Recent advances in enzymatic oxidation of alcohols

Ji Liu et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2018)

Article Biochemical Research Methods

Nanoreactors: Strategies to encapsulate enzyme biocatalysts in virus-like particles

Joshua W. Wilkerson et al.

NEW BIOTECHNOLOGY (2018)

Article Biochemical Research Methods

Evolving biocatalysis to meet bioeconomy challenges and opportunities

Alessandro Pellis et al.

NEW BIOTECHNOLOGY (2018)

Review Biotechnology & Applied Microbiology

Flow Bioreactors as Complementary Tools for Biocatalytic Process Intensification

Lucia Tamborini et al.

TRENDS IN BIOTECHNOLOGY (2018)

Article Chemistry, Multidisciplinary

The Road to Biorenewables: Carbohydrates to Commodity Chemicals

Roger A. Sheldon

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2018)

Article Chemistry, Multidisciplinary

Catalytic Promiscuity of Galactose Oxidase: A Mild Synthesis of Nitriles from Alcohols, Air, and Ammonia

Jan Vilim et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Article Biotechnology & Applied Microbiology

Magnetization of active inclusion bodies: comparison with centrifugation in repetitive biotransformations

Romana Koszagova et al.

MICROBIAL CELL FACTORIES (2018)

Article Multidisciplinary Sciences

Direct production of a genetically-encoded immobilized biodiesel catalyst

Bradley S. Heater et al.

SCIENTIFIC REPORTS (2018)

Article Chemistry, Multidisciplinary

Self-Sufficient Flow-Biocatalysis by Coimmobilization of Pyridoxal 5′-Phosphate and ω-Transaminases onto Porous Carriers

Ana I. Benitez-Mateos et al.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2018)

Article Chemistry, Applied

Biocatalytic N-Acylation of Amines in Water Using an Acyltransferase from Mycobacterium smegmatis

Martina Letizia Contente et al.

ADVANCED SYNTHESIS & CATALYSIS (2018)

Review Chemistry, Multidisciplinary

Extending the application of biocatalysis to meet the challenges of drug development

Paul N. Devine et al.

NATURE REVIEWS CHEMISTRY (2018)

Article Chemistry, Applied

Ester Synthesis in Water: Mycobacterium smegmatis Acyl Transferase for Kinetic Resolutions

Nicolas de Leeuw et al.

ADVANCED SYNTHESIS & CATALYSIS (2018)

Review Chemistry, Multidisciplinary

Role of Biocatalysis in Sustainable Chemistry

Roger A. Sheldon et al.

CHEMICAL REVIEWS (2018)

Review Chemistry, Multidisciplinary

Artificial Biocatalytic Linear Cascades for Preparation of Organic Molecules

Joerg H. Schrittwieser et al.

CHEMICAL REVIEWS (2018)

Review Chemistry, Multidisciplinary

Key Green Chemistry research areas from a pharmaceutical manufacturers' perspective revisited

Marian C. Bryan et al.

GREEN CHEMISTRY (2018)

Article Chemistry, Physical

Opportunities and challenges for combining chemo- and biocatalysis

Florian Rudroff et al.

NATURE CATALYSIS (2018)

Article Biochemistry & Molecular Biology

Asymmetric Ketone Reduction by Imine Reductases

Maike Lenz et al.

CHEMBIOCHEM (2017)

Article Chemistry, Multidisciplinary

Amine dehydrogenases: efficient biocatalysts for the reductive amination of carbonyl compounds

Tanja Knaus et al.

GREEN CHEMISTRY (2017)

Review Green & Sustainable Science & Technology

Waste bio-refineries for the cassava starch industry: New trends and review of alternatives

Antonio Santos Sanchez et al.

RENEWABLE & SUSTAINABLE ENERGY REVIEWS (2017)

Article Chemistry, Analytical

Microfluidic reactors with immobilized enzymes-Characterization, dividing, perspectives

Kinga Meller et al.

SENSORS AND ACTUATORS B-CHEMICAL (2017)

Review Biotechnology & Applied Microbiology

Recent advances in imine reductase-catalyzed reactions

Maike Lenz et al.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2017)

Article Cell Biology

Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology

Randall A. Hughes et al.

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY (2017)

Article Chemistry, Medicinal

Biocatalysis in the Pharmaceutical Industry: The Need for Speed

Matthew D. Truppo

ACS MEDICINAL CHEMISTRY LETTERS (2017)

Article Multidisciplinary Sciences

A synthetic biochemistry platform for cell free production of monoterpenes from glucose

Tyler P. Korman et al.

NATURE COMMUNICATIONS (2017)

Article Chemistry, Physical

Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1

Gao-Wei Zheng et al.

ACS CATALYSIS (2017)

Review Agricultural Engineering

Strategies for design of improved biocatalysts for industrial applications

Aravind Madhavan et al.

BIORESOURCE TECHNOLOGY (2017)

Review Chemistry, Multidisciplinary

Cascades in Compartments: En Route to Machine-Assisted Biotechnology

Kersten S. Rabe et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Review Chemistry, Multidisciplinary

The Hitchhiker's Guide to Flow ChemistryII

Matthew B. Plutschack et al.

CHEMICAL REVIEWS (2017)

Review Chemistry, Multidisciplinary

A Retrosynthesis Approach for Biocatalysis in Organic Synthesis

Rodrigo O. M. A. de Souza et al.

CHEMISTRY-A EUROPEAN JOURNAL (2017)

Article Computer Science, Interdisciplinary Applications

Bioprocess intensification for the effective production of chemical products

John M. Woodley

COMPUTERS & CHEMICAL ENGINEERING (2017)

Review Biochemistry & Molecular Biology

Imine reductases (IREDs)

Juan Mangas-Sanchez et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2017)

Article Chemistry, Multidisciplinary

Photoelectrochemical Reduction of Carbon Dioxide to Methanol through a Highly Efficient Enzyme Cascade

Su Keun Kuk et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Article Chemistry, Multidisciplinary

InspIRED by Nature: NADPH-Dependent Imine Reductases (IREDs) as Catalysts for the Preparation of Chiral Amines

Gideon Grogan et al.

CHEMISTRY-A EUROPEAN JOURNAL (2016)

Article Biochemistry & Molecular Biology

A Rationally Designed Connector for Assembly of Protein-Functionalized DNA Nanostructures

Katja J. Kossmann et al.

CHEMBIOCHEM (2016)

Article Biochemistry & Molecular Biology

A Three-Enzyme Pathway with an Optimised Geometric Arrangement to Facilitate Substrate Transfer

Minghui Liu et al.

CHEMBIOCHEM (2016)

Article Chemistry, Multidisciplinary

New Concepts for Increasing the Efficiency in Directed Evolution of Stereoselective Enzymes

Zhoutong Sun et al.

CHEMISTRY-A EUROPEAN JOURNAL (2016)

Review Biochemistry & Molecular Biology

Enzyme Engineering for In Situ Immobilization

Fabian B. H. Rehm et al.

MOLECULES (2016)

Article Multidisciplinary Sciences

A synthetic pathway for the fixation of carbon dioxide in vitro

Thomas Schwander et al.

SCIENCE (2016)

Article Multidisciplinary Sciences

Forward design of a complex enzyme cascade reaction

Christoph Hold et al.

NATURE COMMUNICATIONS (2016)

Article Biotechnology & Applied Microbiology

Microbial bioinformatics 2020

Mark J. Pallen

MICROBIAL BIOTECHNOLOGY (2016)

Review Chemistry, Applied

Biocatalytic Imine Reduction and Reductive Amination of Ketones

Joerg H. Schrittwieser et al.

ADVANCED SYNTHESIS & CATALYSIS (2015)

Review Chemistry, Multidisciplinary

Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution

Hans Renata et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

Review Chemistry, Multidisciplinary

Continuous-Flow TechnologyA Tool for the Safe Manufacturing of Active Pharmaceutical Ingredients

Bernhard Gutmann et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

Review Biochemical Research Methods

HaloTag Technology: A Versatile Platform for Biomedical Applications

Christopher G. England et al.

BIOCONJUGATE CHEMISTRY (2015)

Review Biotechnology & Applied Microbiology

Redesigning alcohol dehydrogenases/reductases for more efficient biosynthesis of enantiopure isomers

Rongzhen Zhang et al.

BIOTECHNOLOGY ADVANCES (2015)

Review Genetics & Heredity

Insights from 20 years of bacterial genome sequencing

Miriam Land et al.

FUNCTIONAL & INTEGRATIVE GENOMICS (2015)

Review Microbiology

Twenty years of bacterial genome sequencing

Nicholas J. Loman et al.

NATURE REVIEWS MICROBIOLOGY (2015)

Article Chemistry, Applied

Process Development of CuI/ABNO/NMI-Catalyzed Aerobic Alcohol Oxidation

Janelle E. Steves et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2015)

Article Multidisciplinary Sciences

Conversion of alcohols to enantiopure amines through dual-enzyme hydrogen-borrowing cascades

Francesco G. Mutti et al.

SCIENCE (2015)

Review Biochemistry & Molecular Biology

Recent trends and valorization of immobilization strategies and ligninolytic enzymes by industrial biotechnology

Muhammad Asgher et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2014)

Article Chemistry, Applied

Development of a Multi-Step Synthesis and Workup Sequence for an Integrated, Continuous Manufacturing Process of a Pharmaceutical

Patrick L. Heider et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2014)

Article Chemistry, Organic

Recent topics in catalytic asymmetric hydrogenation of ketones

Masahiro Yoshimura et al.

TETRAHEDRON LETTERS (2014)

Article Chemistry, Applied

The Evolution of an Amine Dehydrogenase Biocatalyst for the Asymmetric Production of Chiral Amines

Michael J. Abrahamson et al.

ADVANCED SYNTHESIS & CATALYSIS (2013)

Review Chemistry, Multidisciplinary

Immobilised enzymes in biorenewables production

Maurice C. R. Franssen et al.

CHEMICAL SOCIETY REVIEWS (2013)

Article Biochemistry & Molecular Biology

Biocatalytic retrosynthesis

Nicholas J. Turner et al.

NATURE CHEMICAL BIOLOGY (2013)

Article Chemistry, Applied

Advances in the Process Development of Biocatalytic Processes

Par Tufvesson et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2013)

Article Multidisciplinary Sciences

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

Pedro S. Coelho et al.

SCIENCE (2013)

Review Biochemistry & Molecular Biology

Biocatalytic Synthesis of Chiral Alcohols and Amino Acids for Development of Pharmaceuticals

Ramesh N. Patel

BIOMOLECULES (2013)

Article Chemistry, Multidisciplinary

Nanoreactors by Programmed Enzyme Encapsulation Inside the Capsid of the Bacteriophage P22

Dustin P. Patterson et al.

ACS NANO (2012)

Article Multidisciplinary Sciences

Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin

Bijan Zakeri et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Review Multidisciplinary Sciences

Valorization of Biomass: Deriving More Value from Waste

Christopher O. Tuck et al.

SCIENCE (2012)

Review Biotechnology & Applied Microbiology

Effects of shear on proteins in solution

C. R. Thomas et al.

BIOTECHNOLOGY LETTERS (2011)

Review Biochemistry & Molecular Biology

Practical chiral alcohol manufacture using ketoreductases

Gjalt W. Huisman et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2010)

Article Chemistry, Multidisciplinary

A green-by-design biocatalytic process for atorvastatin intermediate

Steven K. Ma et al.

GREEN CHEMISTRY (2010)

Review Genetics & Heredity

APPLICATIONS OF NEXT-GENERATION SEQUENCING Sequencing technologies - the next generation

Michael L. Metzker

NATURE REVIEWS GENETICS (2010)

Article Multidisciplinary Sciences

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

Christopher K. Savile et al.

SCIENCE (2010)

Review Chemistry, Multidisciplinary

Function-oriented synthesis, step economy, and drug design

Paul A. Wender et al.

ACCOUNTS OF CHEMICAL RESEARCH (2008)

Review Chemistry, Multidisciplinary

E factors, green chemistry and catalysis: an odyssey

Roger A. Sheldon

CHEMICAL COMMUNICATIONS (2008)

Article Biochemistry & Molecular Biology

Structure of a novel enzyme that catalyzes acyl transfer to alcohols in aqueous conditions

Irimpan Mathews et al.

BIOCHEMISTRY (2007)

Article Biotechnology & Applied Microbiology

Improving catalytic function by ProSAR-driven enzyme evolution

Richard J. Fox et al.

NATURE BIOTECHNOLOGY (2007)

Article Biochemistry & Molecular Biology

Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide

Marion B. Ansorge-Schumacher et al.

FEBS JOURNAL (2006)

Article Chemistry, Multidisciplinary

Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test

MT Reetz et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2005)

Review Chemistry, Applied

Concepts of nature in organic synthesis: Cascade catalysis and multistep conversions in concert

A Bruggink et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2003)

Article Chemistry, Multidisciplinary

Application of directed evolution in the development of enantioselective enzymes

MT Reetz

PURE AND APPLIED CHEMISTRY (2000)